海马星形胶质细胞中的烟碱型胆碱能信号传导涉及细胞内钙库的钙诱导钙释放。
Nicotinic cholinergic signaling in hippocampal astrocytes involves calcium-induced calcium release from intracellular stores.
作者信息
Sharma G, Vijayaraghavan S
机构信息
Department of Physiology and Biophysics, University of Colorado Health Sciences Center, Denver, CO 80262, USA.
出版信息
Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):4148-53. doi: 10.1073/pnas.071540198. Epub 2001 Mar 20.
Abstract
In this report we provide evidence that neuronal nicotinic acetylcholine receptors (nAChRs) are present on hippocampal astrocytes and their activation produces rapid currents and calcium transients. Our data indicate that these responses obtained from astrocytes are primarily mediated by an AChR subtype that is functionally blocked by alpha-bungarotoxin (alpha Bgt) and contains the alpha7 subunit (alpha Bgt-AChRs). Furthermore, their action is unusual in that they effectively increase intracellular free calcium concentrations by activating calcium-induced calcium release from intracellular stores, triggered by influx through the receptor channels. These results reveal a mechanism by which alpha Bgt-AChRs on astrocytes can efficiently modulate calcium signaling in the central nervous system in a manner distinct from that observed with these receptors on neurons.
摘要
在本报告中，我们提供证据表明，海马星形胶质细胞上存在神经元烟碱型乙酰胆碱受体（nAChRs），其激活可产生快速电流和钙瞬变。我们的数据表明，从星形胶质细胞获得的这些反应主要由一种AChR亚型介导，该亚型在功能上被α-银环蛇毒素（αBgt）阻断，并含有α7亚基（αBgt-AChRs）。此外，它们的作用不同寻常，因为它们通过激活由受体通道内流触发的细胞内钙库的钙诱导钙释放，有效地增加细胞内游离钙浓度。这些结果揭示了一种机制，通过该机制星形胶质细胞上的αBgt-AChRs能够以不同于神经元上这些受体的方式有效调节中枢神经系统中的钙信号传导。
相似文献
1
Nicotinic cholinergic signaling in hippocampal astrocytes involves calcium-induced calcium release from intracellular stores.海马星形胶质细胞中的烟碱型胆碱能信号传导涉及细胞内钙库的钙诱导钙释放。
Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):4148-53. doi: 10.1073/pnas.071540198. Epub 2001 Mar 20.
2
Diversity of nicotinic acetylcholine receptors in rat hippocampal neurons. IV. Regulation by external Ca++ of alpha-bungarotoxin-sensitive receptor function and of rectification induced by internal Mg++.大鼠海马神经元中烟碱型乙酰胆碱受体的多样性。IV. 细胞外钙离子对α-银环蛇毒素敏感性受体功能及细胞内镁离子诱导的整流作用的调节。
J Pharmacol Exp Ther. 1996 Apr;277(1):432-44.
3
Novel subpopulation of neuronal acetylcholine receptors among those binding alpha-bungarotoxin.在与α-银环蛇毒素结合的神经元乙酰胆碱受体中存在新型亚群。
Mol Pharmacol. 1995 Apr;47(4):717-25.
4
Modulation of alpha7 nicotinic receptor-mediated calcium influx by nicotinic agonists.烟碱激动剂对α7烟碱型受体介导的钙内流的调节作用。
Mol Pharmacol. 1997 Mar;51(3):499-506.
5
Differential effects of chronic drug treatment on alpha3* and alpha7 nicotinic receptor binding sites, in hippocampal neurones and SH-SY5Y cells.慢性药物治疗对海马神经元和SH-SY5Y细胞中α3*和α7烟碱型受体结合位点的不同影响。
Br J Pharmacol. 2001 Aug;133(8):1286-95. doi: 10.1038/sj.bjp.0704207.
6
Dendritic Ca2+ signalling due to activation of alpha 7-containing nicotinic acetylcholine receptors in rat hippocampal neurons.大鼠海马神经元中含α7的烟碱型乙酰胆碱受体激活引起的树突状Ca2+信号传导。
J Physiol. 2007 Jul 15;582(Pt 2):597-611. doi: 10.1113/jphysiol.2007.135319. Epub 2007 May 17.
7
An increase in intracellular free calcium ions by nicotinic acetylcholine receptors in a single cultured rat cortical astrocyte.单个培养的大鼠皮质星形胶质细胞中烟碱型乙酰胆碱受体使细胞内游离钙离子增加。
J Neurosci Res. 2005 Feb 15;79(4):535-44. doi: 10.1002/jnr.20398.
8
Differential coupling of alpha7 and non-alpha7 nicotinic acetylcholine receptors to calcium-induced calcium release and voltage-operated calcium channels in PC12 cells.α7和非α7烟碱型乙酰胆碱受体与PC12细胞中钙诱导的钙释放及电压门控钙通道的差异偶联
J Neurochem. 2007 Feb;100(4):1089-96. doi: 10.1111/j.1471-4159.2006.04273.x. Epub 2006 Dec 20.
9
Identification of regions involved in the binding of alpha-bungarotoxin to the human alpha7 neuronal nicotinic acetylcholine receptor using synthetic peptides.利用合成肽鉴定参与α-银环蛇毒素与人α7神经元烟碱型乙酰胆碱受体结合的区域。
Biochem J. 2003 Jun 1;372(Pt 2):543-54. doi: 10.1042/BJ20021537.
10
Nicotinic receptor-induced apoptotic cell death of hippocampal progenitor cells.烟碱型受体诱导的海马祖细胞凋亡性细胞死亡。
J Neurosci. 1998 Sep 1;18(17):6871-81. doi: 10.1523/JNEUROSCI.18-17-06871.1998.
引用本文的文献
1
Neuronal and Glial α7 Nicotinic Acetylcholine Receptors: Role in Alzheimer's Disease Pathophysiology.神经元和神经胶质细胞的α7烟碱型乙酰胆碱受体：在阿尔茨海默病病理生理学中的作用
Life (Basel). 2025 Jun 28;15(7):1032. doi: 10.3390/life15071032.
2
Nicotine Attenuates Pathogenesis of Parkinson's Disease via α7-nAChR-Mediated Lipid Metabolic Reprogramming and Anti-inflammatory Signaling.尼古丁通过α7-烟碱型乙酰胆碱受体介导的脂质代谢重编程和抗炎信号减轻帕金森病的发病机制。
Mol Neurobiol. 2025 Jun 9. doi: 10.1007/s12035-025-05124-9.
3
Structural basis for allosteric agonism of human α7 nicotinic acetylcholine receptors.人类α7烟碱型乙酰胆碱受体变构激动作用的结构基础。
Cell Discov. 2025 Apr 8;11(1):35. doi: 10.1038/s41421-025-00788-y.
4
Computational modeling of the relationship between morphological heterogeneity and functional responses in mouse hippocampal astrocytes.小鼠海马星形胶质细胞形态异质性与功能反应之间关系的计算建模
Front Cell Neurosci. 2024 Oct 17;18:1474948. doi: 10.3389/fncel.2024.1474948. eCollection 2024.
5
Astrocyte-Neuron Interactions in Substance Use Disorders.星形细胞-神经元相互作用在物质使用障碍中的作用。
Adv Neurobiol. 2024;39:165-191. doi: 10.1007/978-3-031-64839-7_7.
6
Exposing the latent phenotype of Gulf War Illness: examination of the mechanistic mediators of cognitive dysfunction.揭示海湾战争综合征的潜在表型：认知功能障碍的机制中介因素研究。
Front Immunol. 2024 Jun 11;15:1403574. doi: 10.3389/fimmu.2024.1403574. eCollection 2024.
7
AKT2 modulates astrocytic nicotine responses .AKT2调节星形胶质细胞对尼古丁的反应。
bioRxiv. 2024 Jun 1:2024.05.31.596856. doi: 10.1101/2024.05.31.596856.
8
Fluorocitrate inhibition of astrocytes reduces nicotine self-administration and alters extracellular levels of glutamate and dopamine within the nucleus accumbens in male wistar rats.氟柠檬酸抑制星形胶质细胞可减少雄性 wistar 大鼠的尼古丁自我给药，并改变伏隔核内的谷氨酸和多巴胺的细胞外水平。
Neuropharmacology. 2024 Sep 1;255:110001. doi: 10.1016/j.neuropharm.2024.110001. Epub 2024 May 13.
9
Translational implications of CHRFAM7A, an elusive human-restricted fusion gene.CHRFAM7A的转化意义，一种难以捉摸的人类特异性融合基因。
Mol Psychiatry. 2024 Apr;29(4):1020-1032. doi: 10.1038/s41380-023-02389-1. Epub 2024 Jan 10.
10
Inhibition of nicotinic acetylcholine receptors by oligoarginine peptides and polyamine-related compounds.寡聚精氨酸肽和多胺相关化合物对烟碱型乙酰胆碱受体的抑制作用。
Front Pharmacol. 2023 Dec 15;14:1327603. doi: 10.3389/fphar.2023.1327603. eCollection 2023.
本文引用的文献
1
Long-term potentiation of excitatory inputs to brain reward areas by nicotine.尼古丁对大脑奖赏区域兴奋性输入的长期增强作用。
Neuron. 2000 Aug;27(2):349-57. doi: 10.1016/s0896-6273(00)00042-8.
2
Expression and channel properties of alpha-bungarotoxin-sensitive acetylcholine receptors on chick ciliary and choroid neurons.鸡睫状和脉络膜神经元上α-银环蛇毒素敏感型乙酰胆碱受体的表达及通道特性
J Neurophysiol. 2000 Sep;84(3):1314-29. doi: 10.1152/jn.2000.84.3.1314.
3
Calcium signalling mediated by the 9 acetylcholine receptor in a cochlear cell line from the Immortomouse.由永生小鼠的耳蜗细胞系中的9型乙酰胆碱受体介导的钙信号传导。
J Physiol. 2000 Aug 15;527(Pt 1):49-54. doi: 10.1111/j.1469-7793.2000.t01-1-00049.x.
4
Physiological astrocytic calcium levels stimulate glutamate release to modulate adjacent neurons.生理性星形胶质细胞钙水平刺激谷氨酸释放以调节相邻神经元。
Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8629-34. doi: 10.1073/pnas.97.15.8629.
5
Unloading and refilling of two classes of spatially resolved endoplasmic reticulum Ca(2+) stores in astrocytes.星形胶质细胞中两类空间分辨的内质网Ca(2+)储存库的卸载和再填充。
Glia. 2000 Jul;31(1):15-28. doi: 10.1002/(sici)1098-1136(200007)31:1<15::aid-glia20>3.0.co;2-h.
6
Mice homozygous for the L250T mutation in the alpha7 nicotinic acetylcholine receptor show increased neuronal apoptosis and die within 1 day of birth.α7烟碱型乙酰胆碱受体L250T突变纯合子小鼠表现出神经元凋亡增加，并在出生后1天内死亡。
J Neurochem. 2000 May;74(5):2154-66. doi: 10.1046/j.1471-4159.2000.0742154.x.
7
Smoking and schizophrenia: abnormal nicotinic receptor expression.吸烟与精神分裂症：烟碱型受体表达异常
Eur J Pharmacol. 2000 Mar 30;393(1-3):237-42. doi: 10.1016/s0014-2999(00)00035-2.
8
Ion channels in glial cells.神经胶质细胞中的离子通道。
Brain Res Brain Res Rev. 2000 Apr;32(2-3):380-412. doi: 10.1016/s0165-0173(99)00093-4.
9
Extracellular calcium regulates responses of both alpha3- and alpha7-containing nicotinic receptors on chick ciliary ganglion neurons.细胞外钙调节鸡睫状神经节神经元上含α3和α7的烟碱样受体的反应。
J Neurophysiol. 1999 Sep;82(3):1124-32. doi: 10.1152/jn.1999.82.3.1124.
10
Three-dimensional relationships between hippocampal synapses and astrocytes.海马体突触与星形胶质细胞之间的三维关系。
J Neurosci. 1999 Aug 15;19(16):6897-906. doi: 10.1523/JNEUROSCI.19-16-06897.1999.
Zotero 插件