Kiknadze I I, Vlasova I E, Sherudilo A I
Cell Differ. 1975 Mar;3(6):323-34. doi: 10.1016/0045-6039(75)90001-9.
Relative DNA content during the polytenization of the salivary gland nuclei of Chironomus thummi was measured by cytophotometric and cytofluorometric methods. To estimate the degree of polyteny, the DNA content was calculated in hemocyte and spermatocyte nuclei. Chromosome polytenization is associated with 10 to 12 replication rounds. There are 4-5 replication rounds in 1st instar, 2-3 rounds in 2nd instar; 3rd and 4th instars have 1-2 rounds each. From early postembryonic development, larvae already have salivary gland nuclei representing two polyteny classes (2-3 - 2-4C); A similar heterogeneity is retained in all instars. The approximate DNA content is 0.51-0.58 picogram per diploid set.
采用细胞光度法和细胞荧光光度法测定了图氏摇蚊唾液腺细胞核多线化过程中的相对DNA含量。为了估计多线化程度，计算了血细胞和精母细胞核中的DNA含量。染色体多线化与10至12次复制周期相关。一龄幼虫有4 - 5次复制周期，二龄幼虫有2 - 3次；三龄和四龄幼虫各有1 - 2次。从胚胎后期发育早期开始，幼虫的唾液腺细胞核就已经代表了两个多线化类别（2 - 3 - 2 - 4C）；所有龄期都保留了类似的异质性。每个二倍体组的DNA含量约为0.51 - 0.58皮克。
