Arakawa T, Prestrelski S J, Kenney W C, Carpenter J F
Amgen Inc., Amgen Center, Thousand Oaks, CA, USA.
Adv Drug Deliv Rev. 2001 Mar 1;46(1-3):307-26. doi: 10.1016/s0169-409x(00)00144-7.
Proteins are marginally stable and, hence, are readily denatured by various stresses encountered in solution, or in the frozen or dried states. Various additives are known to minimize damage and enhance the stability of proteins. This review discusses the current knowledge of the mechanisms by which these additives stabilize proteins against acute stresses, and also the various factors to be considered for long-term storage of proteins in solution.
蛋白质的稳定性较差，因此，在溶液中、冷冻或干燥状态下遇到的各种应力很容易使其变性。已知各种添加剂可将蛋白质的损伤降至最低并增强其稳定性。本文综述了关于这些添加剂使蛋白质抵抗急性应激的稳定机制的现有知识，以及在溶液中长期储存蛋白质时需要考虑的各种因素。
