犬甲状旁腺中膜结合核糖体和游离核糖体的蛋白质合成活性。

Protein synthetic activity of membrane-bound and free ribosomes from parathyroid glands of dogs.

Alford F P, Cook P S, Swenson R S, Reaven G M

Clin Sci Mol Med. 1975 May;48(5):373-8. doi: 10.1042/cs0480373.

Abstract

Membrane-bound and free polyribosomes were isolated from parathyroid glands of normal dogs by a discontinuous density-gradient technique. 2. The conditions necessary for optimum incorporation by bound and free ribosomes of [3-H]-phenylalanine into protein were determined for assays in vitro directed by both endogenous messenger ribonucleic acid (mRNA) and polyuridylic acid [poly(U)]. 3. When the specific cofactors were available in optimum amounts, the rate of incorporation of amino acids into protein was directly proportional to the number of ribosomes present. This applied to assays directed by endogenous mRNA and poly-(U). 4. The results indicate that it is possible to isolate and directly study the protein synthetic activity of membrane-bound and free parathyroid ribosomes.

摘要

采用不连续密度梯度技术，从正常犬的甲状旁腺中分离出膜结合多核糖体和游离多核糖体。2. 确定了在由内源性信使核糖核酸（mRNA）和聚尿苷酸[poly(U)]指导的体外测定中，膜结合核糖体和游离核糖体将[3-H] - 苯丙氨酸最佳掺入蛋白质所需的条件。3. 当特定辅因子以最佳量存在时，氨基酸掺入蛋白质的速率与存在的核糖体数量成正比。这适用于由内源性mRNA和聚（U）指导的测定。4. 结果表明，有可能分离并直接研究膜结合和游离甲状旁腺核糖体的蛋白质合成活性。