Chen G H, Tang S J, Chen C S, Jiang S T
Deaprtment of Food Science and Institute of Marine Biotechnology, National Taiwan Ocean University, Keelung, Taiwan.
J Agric Food Chem. 2001 Feb;49(2):641-6. doi: 10.1021/jf000992s.
A high level of the secreted form of recombinant chicken cystatin was expressed in Pichia pastoris X-33 by chromosomal integration of multiple copies of an expression cassette containing chicken cystatin under the control of glyceraldehyde-3-phosphate dehydrogenase promoter. The inhibition ability of the recombinant for papain-like proteinase was found to correspond to those of natural chicken cystatin. The recombinant cystatin substantially inhibited the proteolysis of myosin and gel softening, which consequently improved the gel properties of mackerel surimi.
通过将含有鸡半胱氨酸蛋白酶抑制剂的表达盒多拷贝整合到染色体中，在毕赤酵母X-33中表达了高水平的重组鸡半胱氨酸蛋白酶抑制剂分泌形式。发现该重组体对木瓜蛋白酶样蛋白酶的抑制能力与天然鸡半胱氨酸蛋白酶抑制剂的抑制能力相当。重组半胱氨酸蛋白酶抑制剂显著抑制了肌球蛋白的蛋白水解和凝胶软化，从而改善了鲭鱼鱼糜的凝胶特性。
