[蓖麻油饮食对妊娠大鼠前列腺素E2合成的影响]
[Effects of castor oil-diet on the synthesis of prostaglandin E2 in pregnant rats].
作者信息
Gao J, Sun N, Wang F
机构信息
Chinese Academy of Medical Sciences, Peking Union Medical College Peking Union Medical College Hospital, Beijing 100730.
出版信息
Zhonghua Fu Chan Ke Za Zhi. 1999 Mar;34(3):147-9.
OBJECTIVE
To determine effects of castor oil-diet on the synthesis of prostaglandin E2(PGE2) and explore the mechanism of labor induced by castor oil-diet in pregnant rats.
METHODS
Pregnant rats were gavaged castor oil-diet in 18 and 19 days of gestation. At the time of death, blood of portal and peripheral veins, intestinal mucosa, amnion, amniotic cells and placenta were collected, and the tissues were cultured in the presence of ricinoleic acid or indomethacin. The concentrations of PGE2 in the media or blood were measured by RIA methods.
RESULTS
The PGE2 levels in the portal vein increased, while the PGE2 levels in peripheral blood had no changes significantly, the PGE2 levels in the tissues of the intestinal mucosa, placenta, amnion and amniotic cells were increased significantly; Ricinoleic acid stimulated the synthesis of PGE2 in the above tissues in vitro, which had the positive correlations with the dose of ricinoleic acid and its lasting time. Indomethacin inhibited the synthesis of PGE2 in-vitro.
CONCLUSION
The increased synthesis of PGE2 in the intrauterine tissues is a key of the initiation of labor induced by castor oil-diet, and ricinoleic acid in castor oil-diet might be the active component which induced the initiator of labor.
目的
探讨蓖麻油餐对前列腺素E2（PGE2）合成的影响，以阐明蓖麻油餐诱发孕鼠分娩的机制。
方法
妊娠18、19天的孕鼠给予蓖麻油餐。处死时，采集门静脉血、外周静脉血、肠黏膜、羊膜、羊膜细胞和胎盘组织，将组织在蓖麻油酸或吲哚美辛存在的情况下进行培养。用放射免疫分析法测定培养基或血液中PGE2的浓度。
结果
门静脉血中PGE2水平升高，外周血中PGE2水平无明显变化，肠黏膜、胎盘、羊膜和羊膜细胞组织中PGE2水平显著升高；蓖麻油酸在体外刺激上述组织中PGE2的合成，且与蓖麻油酸剂量和作用时间呈正相关。吲哚美辛在体外抑制PGE2的合成。
结论
宫内组织中PGE2合成增加是蓖麻油餐诱发分娩的关键，蓖麻油餐中的蓖麻油酸可能是诱发分娩的活性成分。
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