白果内酯对β-淀粉样肽25-35诱导的PC12细胞毒性的保护作用。

Protective effects of bilobalide on amyloid beta-peptide 25-35-induced PC12 cell cytotoxicity.

作者信息

Zhou L J, Song W, Zhu X Z, Chen Z L, Yin M L, Cheng X F

机构信息

Department of Pharmacology I, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China.

出版信息

Acta Pharmacol Sin. 2000 Jan;21(1):75-9.

PMID:11263252

Abstract

AIM

To study the effect of bilobalide, a terpene extracted from the leaves of Ginkgo biloba, on beta-amyloid peptide fragment 25-35 (A beta 25-35)-induced PC12 cell cytotoxicity.

METHODS

3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assay were used to measure the viability of PC12 cells. Thiobarbituric acid-reactive substances were measured to determine lipid peroxidation of cells. Antioxidant enzymes in PC12 cells were detected.

RESULTS

Treatment of PC12 cells with A beta 25-35 (100 mumol.L-1) for 24 h caused a great decrease in cell viability (P < 0.01 compared with control). Bilobalide 25-100 mumol.L-1 dose-dependently attenuated the cytotoxic effect of A beta 25-35. Bilobalide also inhibited A beta 25-35 (100 mumol.L-1)-induced elevation of lipid peroxidation and decline of antioxidant enzyme activities.

CONCLUSION

Bilobalide protected PC12 cells from A beta 25-35-induced cytotoxicity.

摘要

目的

研究从银杏叶中提取的萜类化合物白果内酯对β-淀粉样肽片段25 - 35（Aβ25 - 35）诱导的PC12细胞毒性的影响。

方法

采用3 - [4,5 - 二甲基噻唑 - 2 - 基] - 2,5 - 二苯基四氮唑溴盐和乳酸脱氢酶测定法检测PC12细胞的活力。通过测定硫代巴比妥酸反应性物质来确定细胞的脂质过氧化情况。检测PC12细胞中的抗氧化酶。

结果

用Aβ25 - 35（100μmol·L-1）处理PC12细胞24小时导致细胞活力大幅下降（与对照组相比，P < 0.01）。25 - 100μmol·L-1的白果内酯剂量依赖性地减弱了Aβ25 - 35的细胞毒性作用。白果内酯还抑制了Aβ25 - 35（100μmol·L-1）诱导的脂质过氧化升高和抗氧化酶活性下降。