巴曲酶对沙鼠脑缺血再灌注损伤的影响。

Influence of batroxobin on cerebral ischemia-reperfusion injury in gerbils.

作者信息

Chen Q, Zeng Y M, Xu P C, Fan J W

机构信息

Department of Anesthesiology, Affiliated Hospital of Xuzhou Medical College, China.

出版信息

Acta Pharmacol Sin. 2000 Feb;21(2):161-4.

PMID:11263264

Abstract

AIM

To study the effects of batroxobin (Bat) on neurons survival, neurobehavioral test, ATP levels and hydroxyl radical outputs in hippocampus during forebrain ischemia-reperfusion in gerbils.

METHODS

The forebrain ischemia was induced by occluding the bilateral common carotid arteries for 10 min in gerbils, and ATP levels and 2, 3-dihydroxybenzoic acid (DHBA) outputs were assayed by HPLC. The neurons survival were assessed by histology, and behavioral tests of gerbils were assessed by open field test.

RESULTS

The number of neurons survival in Ir at d 7 postischemic insult were (7 +/- 4)% of sham-operated gerbils, much less than that in Bat (45 +/- 16)%. The levels of explore activities of ischemic gerbils was 175% and 159% of sham-operated gerbils at d 3 and d 6 postischemic insult, much more than that in Bat (120% d 3 and 140% d 6). Hippocampal ATP levels in Ir were 64% of sham-operated gerbils at reperfusion 60 min, much less than that in Bat I and II (82% and 89% respectively). The hippocampal 2,3-DHBA outputs in Ir increased by 4.5 folds of sham-operated gerbils at reperfusion 60 min, but the 2,3-DHBA outputs in Bat I and Bat II were only 2.6 and 2.4 folds respectively.

CONCLUSION

Bat possesses the inhibitory effects on DND and OH. production following cerebral ischemia-reperfusion in gerbils.

摘要

目的

研究巴曲酶（Bat）对沙土鼠前脑缺血再灌注期间海马神经元存活、神经行为学测试、ATP水平及羟自由基生成的影响。

方法

通过阻断沙土鼠双侧颈总动脉10分钟诱导前脑缺血，采用高效液相色谱法测定ATP水平及2,3 -二羟基苯甲酸（DHBA）生成量。通过组织学评估神经元存活情况，采用旷场试验评估沙土鼠的行为学测试。

结果

缺血损伤后第7天，缺血再灌注组（Ir）存活神经元数量为假手术组沙土鼠的（7±4）%，远低于巴曲酶组（Bat）的（45±16）%。缺血沙土鼠在缺血损伤后第3天和第6天的探索活动水平分别为假手术组的175%和159%，远高于巴曲酶组（第3天为120%，第6天为140%）。再灌注60分钟时，缺血再灌注组海马ATP水平为假手术组的64%，远低于巴曲酶I组和II组（分别为82%和89%）。再灌注60分钟时，缺血再灌注组海马2,3 - DHBA生成量增加至假手术组的4.5倍，但巴曲酶I组和II组的2,3 - DHBA生成量仅分别为2.6倍和2.4倍。