Wu C, Sun B, Mao B
Department of Respiratory Disease, Xijing Hospital, 4th Military Medical University, Xi'an 710032.
Zhonghua Jie He He Hu Xi Za Zhi. 1998 Jan;21(1):16-8.
To investigate the distribution of substance P(SP) in the lungs of asthmatic guinea pigs and the effects of dexamethasone on its distribution.
Guinea pigs were divided into 3 groups: asthma group, dexamethasone-treated group and control group. Rabbit serum against SP, immunohistochemical ABC method and glucose-DAB-nickel technique for staining and computer image analysis were used in this study.
There was distribution of SP-IR positive fibres in airways in asthma group than the other two groups. The positive fibres, emerged like a string of beads, were present as large fibre bundles in the smooth muscle layer and basement membrane. Additionally, in the asthma group SP-IR positive fibres were detected on the walls of respiratory bronchioles and alveolar ducts in contrast to the other groups. There was no difference in distribution and morphological characteristic between the control group and the dexamethasone-treated group.
Repeated antigen challenges, which cause the allergic inflammation of airways, may result in the growth of SP-containing nerve fibres in the airway and synthesis of SP in neurons. These may be involved in the persistence and exacerbation of airway inflammation in asthma. Dexamethasone can reverse the distribution of SP-IR positive fibres to the normal status in airway walls of asthma guinea pigs.
探讨P物质（SP）在哮喘豚鼠肺组织中的分布及地塞米松对其分布的影响。
将豚鼠分为3组：哮喘组、地塞米松治疗组和对照组。本研究采用抗SP兔血清、免疫组织化学ABC法及葡萄糖-DAB-镍技术进行染色和计算机图像分析。
哮喘组气道内SP免疫反应（SP-IR）阳性纤维的分布多于其他两组。阳性纤维呈串珠状，以大纤维束的形式存在于平滑肌层和基底膜中。此外，与其他组相比，哮喘组在呼吸性细支气管和肺泡管的壁上检测到SP-IR阳性纤维。对照组和地塞米松治疗组在分布和形态特征上无差异。
反复抗原刺激可引起气道过敏性炎症，可能导致气道内含SP神经纤维的生长及神经元内SP的合成。这些可能参与哮喘气道炎症的持续和加重。地塞米松可使哮喘豚鼠气道壁上SP-IR阳性纤维的分布恢复至正常状态。
