Feng G, Zhou Y, Yang Z
Research Institute of Surgery, Daping Hospital, Chongqing 400042.
Zhonghua Jie He He Hu Xi Za Zhi. 1998 Jan;21(1):19-22.
To investigate the changes of thromboxane A2 receptors and the influence of their antagonist phenol red, and to study in molecular level the mechanism of action of thromboxane A2 in acute lung injury.
Acute lung injury model of rats was produced by oleic acid, and phenol red (5 mg/kg, 50 mg/kg) was injected via vein 5 minutes before oleic acid injection. Changes of thromboxane A2 receptors and their mRNA expression in the lung were determined at 6 hours after oleic acid injection.
Down-regulation of thromboxane A2 receptors were observed, but their mRNA transcription increased significantly in the lung after oleic acid injection. The injection of phenol red before oleic acid could induce down-regulation of thromboxane A2 receptors and abolish the increase of their mRNA transcription after oleic acid injection. Phenol red could reduce LBI, RLW and improve PaO2. Histological examination of lung tissues demonstrated that the degree of lung injury had been reduced significantly.
Thromboxane A2 plays an important role plays in pathological processes of acute lung injury. Phenol red in vivo could effectively reduce severity of acute lung injury by blocking thromboxane A2 receptors and regulating the expression of thromboxane A2 receptors.
探讨血栓素A2受体的变化及其拮抗剂酚红的影响，从分子水平研究血栓素A2在急性肺损伤中的作用机制。
采用油酸制备大鼠急性肺损伤模型，在注射油酸前5分钟经静脉注射酚红（5mg/kg、50mg/kg）。于注射油酸后6小时测定肺组织中血栓素A2受体及其mRNA表达的变化。
注射油酸后肺组织中血栓素A2受体下调，但其mRNA转录显著增加。油酸注射前注射酚红可诱导血栓素A2受体下调，并消除油酸注射后其mRNA转录的增加。酚红可降低肺湿干重比、右肺重量，改善动脉血氧分压。肺组织学检查显示肺损伤程度明显减轻。
血栓素A2在急性肺损伤的病理过程中起重要作用。体内酚红可通过阻断血栓素A2受体并调节血栓素A2受体的表达有效减轻急性肺损伤的严重程度。
