[一种利用脂多糖和甲吡酮建立豚鼠运动诱发性哮喘的模型]

[A model for exercise-induced asthma in guinea pigs with lipopolysaccharide and metyrapone].

作者信息

Liang Y, Cai Y, Li L

机构信息

Zhongshan Hospital of Shanghai Medical University, Shanghai 200032.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 1998 Mar;21(3):144-6.

PMID:11263348

Abstract

OBJECTIVE

To investigate the relationship between airway inflammation and exercise-induced asthma (EIA), an experimental model for EIA in guinea pigs was developed.

METHOD

27 guinea pigs was divided into 4 groups. A) LPS + MET exercise group (n = 7) had been pretreated with lipopolysaccharide (LPS 1 mg/kg, i.p.) and metyrapone (MET 50 mg/kg, i.p.) on the first day. Then lung resistance (RL) and dynamic compliance (Cdyn) were measured before and after exercise on the fourth day. B) LPS + MET non exercise group (n = 6) that pretreated with LPS and MET had nonexercise on the 4th day. C) NS exercise group (n = 7) pretreated with normal saline (NS 1 mg/kg, i.p.) on the 1st day. RL and Cdyn were measured before and after exercise on the 4th day. D) NS nonexercise group (n = 7) that pretreated with NS had no exercise on the 4th day.

RESULT

In LPS+MET exercise group RL increased and Cdyn reduced significantly after exercise. In another 3 control groups there were no such changes either in RL or in Cdyn.

CONCLUSION

These observations suggest that the treatment with LPS and MET could make a model for exercise-induced asthma.

摘要

目的

为研究气道炎症与运动诱发性哮喘（EIA）之间的关系，建立了豚鼠EIA实验模型。

方法

将27只豚鼠分为4组。A）脂多糖+甲吡酮运动组（n = 7）在第1天腹腔注射脂多糖（LPS 1 mg/kg）和甲吡酮（MET 50 mg/kg）进行预处理。然后在第4天运动前后测量肺阻力（RL）和动态顺应性（Cdyn）。B）脂多糖+甲吡酮非运动组（n = 6），经脂多糖和甲吡酮预处理，在第4天不运动。C）生理盐水运动组（n = 7）在第1天腹腔注射生理盐水（NS 1 mg/kg）进行预处理。在第4天运动前后测量RL和Cdyn。D）生理盐水非运动组（n = 7），经生理盐水预处理，在第4天不运动。