使用腺相关病毒载体将酪氨酸羟化酶和芳香族L-氨基酸脱羧酶基因共转导至培养的纹状体细胞中。
Cotransduction of tyrosine hydroxylase and aromatic L-amino acid decarboxylase genes into cultured striatal cells using adeno-associated virus vectors.
作者信息
Fan D, Kang D, Ogawa M, Nakano I, Nagatsu T, Kurtzman G J, Ozawa K
机构信息
Department of Neurology, 3rd Hospital, Beijing Medical University, Beijing 100083, China.
出版信息
Chin Med J (Engl). 1998 Dec;111(12):1111-3.
OBJECTIVE
To examine whether tyrosine hydroxylase (TH) and aromatic L-amino acid decarboxylase (AADC) genes can be cotransduced into the same target striatal cells using adeno-associated virus (AAV) vectors, and to determine whether the cotransduction would result in better biochemical change than the TH gene alone.
METHODS
TH and AADC genes were cotransduced into cultured striatal cells with separate AAV vectors. Expressions of TH and AADC were detected by immunocytochemistry; intracellular catecholamine levels were assayed by high-performance liquid chromatography (HPLC).
RESULTS
TH and AADC genes were efficiently cotransduced into the striatal cells. Specifically, the coexpression of TH and AADC resulted in more effective dopamine production compared with the TH gene alone.
CONCLUSION
Using AAV vectors, coexpression of TH and AADC in the striatal cells might be a useful approach to gene therapy for Parkinson's disease.
目的
研究是否可利用腺相关病毒（AAV）载体将酪氨酸羟化酶（TH）基因和芳香族L-氨基酸脱羧酶（AADC）基因共转导至同一目标纹状体细胞中，并确定这种共转导是否比单独转导TH基因能带来更好的生化变化。
方法
使用不同的AAV载体将TH基因和AADC基因共转导至培养的纹状体细胞中。通过免疫细胞化学检测TH和AADC的表达；采用高效液相色谱法（HPLC）测定细胞内儿茶酚胺水平。
结果
TH基因和AADC基因能有效共转导至纹状体细胞中。具体而言，与单独的TH基因相比，TH和AADC的共表达能产生更有效的多巴胺。
结论
利用AAV载体，在纹状体细胞中共表达TH和AADC可能是帕金森病基因治疗的一种有效方法。
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