Grafe T U, Thein J
Zoologie 3, Biozentrum, Am Hubland, Würzburg, Germany.
J Comp Physiol B. 2001 Feb;171(1):69-76. doi: 10.1007/s003600000151.
Rates of oxygen consumption and carbon dioxide release were measured in calling and resting European tree frogs using open-flow-through respirometry. The energetic cost of calling was high with an average of 1.076 ml O2/(g.h) at average call rates of 8,000 calls/h. The maximum factorial metabolic scopes averaged 24 with momentary peak values ranging between 5 and 41. There was a threefold difference in O2-consumption between individual males calling at the same rate. Respiratory quotients indicated that both lipids and carbohydrates were used to fuel calling. Carbohydrates provided the major fuel (69% on average) with dependence on carbohydrates increasing with call rate. In contrast to marathon runners, there was no shift in metabolic substrate use over a calling period of 2-3 h.
利用开放式流通呼吸测定法，对正在鸣叫和休息的欧洲树蛙的耗氧率和二氧化碳释放率进行了测量。鸣叫的能量消耗很高，在平均鸣叫速率为8000次/小时时，平均耗氧量为1.076毫升O₂/（克·小时）。最大阶乘代谢范围平均为24，瞬时峰值在5到41之间。以相同速率鸣叫的雄性个体之间，耗氧量存在三倍的差异。呼吸商表明，脂质和碳水化合物都被用于为鸣叫提供能量。碳水化合物提供了主要能量（平均69%），且随着鸣叫速率的增加，对碳水化合物的依赖也增加。与马拉松运动员不同，在2至3小时的鸣叫期间，代谢底物的使用没有变化。
