Lydon J, Patterson C D
U.S. Department of Agriculture, Agricultural Research Service, Sustainable Agricultural Systems Laboratory, Beltsville, MD 20705, USA.
Lett Appl Microbiol. 2001 Mar;32(3):166-70. doi: 10.1046/j.1472-765x.2001.00882.x.
The present study describes a system based on PCR to distinguish tabtoxin-producing strains of Pseudomonas syringae from other Ps. syringae plant pathogens that produce chlorosis-inducing phytotoxins.
Thirty-two strains of Ps. syringae and related species were examined. Two sets of PCR primers were developed to amplify genes (tblA and tabA) required for tabtoxin production. Only a PCR product of 829 bp or 1020 bp was produced in PCR reactions with the tblA or tabA primer sets, respectively, and cells from tabtoxin-producing pathovars of Pseudomonas syringae. All known non-tabtoxin producing bacterial species failed to produce an amplification product with either primer set.
PCR of genes required for tabtoxin production is a simple, rapid and reliable method for identifying tabtoxin-producing strains of Ps. syringae.
The protocol can effectively distinguish tabtoxin-producing strains of Ps. syringae from other Ps. syringae pathovars and Ps. syringae pv. tabaci strains from other tabtoxin-producing Ps. syringae pathovars.
本研究描述了一种基于聚合酶链反应（PCR）的系统，用于区分丁香假单胞菌中产生烟草毒素的菌株与其他产生引起萎黄病的植物毒素的丁香假单胞菌植物病原体。
检测了32株丁香假单胞菌及相关菌种。开发了两组PCR引物，用于扩增产生烟草毒素所需的基因（tblA和tabA）。在分别使用tblA或tabA引物组与丁香假单胞菌产生烟草毒素的致病型细胞进行的PCR反应中，仅分别产生了829 bp或1020 bp的PCR产物。所有已知不产生烟草毒素的细菌物种均未能用任何一组引物产生扩增产物。
对产生烟草毒素所需基因进行PCR是鉴定丁香假单胞菌中产生烟草毒素菌株的一种简单、快速且可靠的方法。
该方案可以有效地将丁香假单胞菌中产生烟草毒素的菌株与其他丁香假单胞菌致病型以及将丁香假单胞菌烟草致病变种菌株与其他产生烟草毒素的丁香假单胞菌致病型区分开来。
