Manconi F, Markham R, Fraser I S
Department of Obstetrics and Gynaecology, Queen Elizabeth II Research Institute for Mothers and Infants, The University of Sydney, NSW 2006, Australia.
Methods Cell Sci. 2000;22(2-3):89-99. doi: 10.1023/a:1009895723488.
There is considerable divergence of opinion on the best methods for the isolation and in vitro culture of microvascular endothelium. Reports have either only described the isolation without mentioning culture conditions or have not fully defined the cell population being cultured. Even at the close of the 20th Century, the isolation and in vitro culture of endothelial cells of microvasculature origin still proves to be technically difficult and many questions remain. These questions need to be addressed by improvements to current methods of isolation and culture of Endothelial cells. A number of more 'high-tech' approaches to this isolation are being explored currently. Use of a more definitive panel of antibodies for immunocytochemical characterization, should enable a more confident characterization of the endothelial cell preparations cultured in vitro. Cell adhesion molecules such as ELAM and VCAM can be used to assist in determining cell population purity.
关于微血管内皮细胞的分离和体外培养的最佳方法，存在相当大的意见分歧。报告要么只描述了分离过程而未提及培养条件，要么没有充分界定所培养的细胞群体。即使到了20世纪末，源自微血管的内皮细胞的分离和体外培养在技术上仍然困难重重，许多问题依然存在。这些问题需要通过改进当前的内皮细胞分离和培养方法来解决。目前正在探索一些更“高科技”的分离方法。使用一组更具确定性的抗体进行免疫细胞化学表征，应该能够更可靠地鉴定体外培养的内皮细胞制剂。细胞黏附分子如ELAM和VCAM可用于辅助确定细胞群体的纯度。
