Byrne K M, Reinhart G A, Hayek M G
Department of Animal Sciences, Washington State University, P.O. Box 646351, Pullman, WA 99164-6351, USA.
Methods Cell Sci. 2000;22(2-3):191-8. doi: 10.1023/a:1009896109932.
Gating in flow cytometry is used to select subpopulations of cells for analysis. The technique is critical for subsequent analysis in order to select the population, free of debris and unrelated cells. Accurately quantifying subpopulations in clinical cases is necessary for correct diagnosis. Human lymphocytes are selected by backgating on populations of CD45+high CD14- cells. These reagents are not available widely across species. In veterinary medicine, markers to identify lymphocytes are usually limited to T-lymphocyte, CD4, CD8, and B-lymphocyte surface antigens. A standardized gating technique using a T-lymphocyte antibody is described and is applicable across species where limited phenotype markers are available.
流式细胞术中的设门用于选择细胞亚群进行分析。该技术对于后续分析至关重要，以便选择不含碎片和无关细胞的群体。在临床病例中准确量化亚群对于正确诊断是必要的。通过反向设门选择CD45+高CD14-细胞群体来筛选人淋巴细胞。这些试剂在不同物种中并非广泛可得。在兽医学中，用于识别淋巴细胞的标志物通常限于T淋巴细胞、CD4、CD8和B淋巴细胞表面抗原。本文描述了一种使用T淋巴细胞抗体的标准化设门技术，该技术适用于可用表型标志物有限的不同物种。
