Obradović S, Vidić-Danković B, Pejcić-Karapetrović B, Kosec D, Leposavić G
Immunology Research Center Branislav Janković, Belgrade, Yugoslavia.
Pharmazie. 2001 Mar;56(3):235-8.
The study was designed to shed more light on the controversial role of the two main ovarian steroid hormones (i.e. estradiol and progesterone) in shaping the size and phenotypic characteristics of the splenic lymphocyte pool. For this purpose ovariectomized adult rats (OVX) were treated for 14 subsequent days with either estradiol or progesterone (to attain physiological concentrations of the hormones). Afterwards, the splenocyte yield, and overall number of splenocytes bearing TCR alpha beta receptor, CD4 and CD8 coreceptor were evaluated. Fourteen-day-long ovarian hormone deprivation produced an increase in the splenic weight and splenocyte yield (on the account of a rise in the number of TCR alpha beta- cells), although the number of TCR alpha beta+ cells was reduced as a result of a decrease in the size of the CD4+ cell subpopulation. Replacement of either estradiol or progesterone prevented the increase in splenic weight and reduced the splenocyte yield to values significantly lower than that in sham-OVX rats. Both the treatments completely abolished the effect of ovariectomy on the size of TCR alpha beta- cell population, but had differential effects on that of TCR alpha beta+ cell population; estradiol did not affect its size, while progesterone caused a reduction on the account of a decrease in the numbers of both CD4+ and CD8+ cells. The results suggest that: a) estradiol and progesterone have similar effects on the size of the splenic B cell population and that replacement of either estradiol or progesterone can prevent the effects of ovariectomy on the size of this population and b) estradiol does not affect while progesterone reduces the size of splenic T cell population. Thus, replacement of none of them is able to compensate the removal of gonads.
该研究旨在更深入地了解两种主要卵巢甾体激素（即雌二醇和孕酮）在塑造脾淋巴细胞库的大小和表型特征方面的争议性作用。为此，对成年去卵巢大鼠（OVX）连续14天给予雌二醇或孕酮治疗（以达到激素的生理浓度）。之后，评估脾细胞产量以及携带TCRαβ受体、CD4和CD8共受体的脾细胞总数。为期14天的卵巢激素剥夺导致脾脏重量和脾细胞产量增加（这是由于TCRαβ-细胞数量增加），尽管由于CD4+细胞亚群大小的减少，TCRαβ+细胞数量减少。给予雌二醇或孕酮均可防止脾脏重量增加，并使脾细胞产量降低至显著低于假手术去卵巢大鼠的值。两种治疗均完全消除了去卵巢对TCRαβ-细胞群体大小的影响，但对TCRαβ+细胞群体的影响不同；雌二醇不影响其大小，而孕酮由于CD4+和CD8+细胞数量减少导致其大小减小。结果表明：a）雌二醇和孕酮对脾B细胞群体大小有相似影响，给予雌二醇或孕酮均可防止去卵巢对该群体大小的影响；b）雌二醇不影响脾T细胞群体大小，而孕酮会使其减小。因此，单独给予它们均无法补偿性腺切除的影响。
