Zhou H, Hickford J G
Animal and Food Sciences Division, Lincoln University, Canterbury, New Zealand.
Vet Microbiol. 2001 Apr 19;79(4):367-74. doi: 10.1016/s0378-1135(00)00385-0.
Dichelobacter nodosus fimbrial subunit gene (fimA) from a serotype M strain (M-SPAHL) was investigated in this study. A primer set targeting the relatively conserved fimA regions and based on the published sequence from Nepalese serogroup M isolates (Nepalese M), failed to amplify the fimA of M-SPAHL. However, when the downstream primer was substituted with a primer that is specific for other serogroups of D. nodosus, the fimA was successfully amplified. Cloning followed by DNA sequencing, revealed that the M-SPAHL fimA was different to the Nepalese M fimA. The predicted amino acid sequence of the M-SPAHL fimA did not show homology to any known serogroups or serotypes. The most similar sequence was from serotype F1, and not Nepalese M. The consequences of serological relatedness and sequence dissimilarity are discussed.
本研究对来自血清型M菌株（M-SPAHL）的结节双歧杆菌菌毛亚基基因（fimA）进行了调查。一套基于尼泊尔血清群M分离株（尼泊尔M）已发表序列、靶向相对保守的fimA区域的引物，未能扩增出M-SPAHL的fimA。然而，当用针对结节双歧杆菌其他血清群的特异性引物替换下游引物时，fimA成功扩增。克隆后进行DNA测序，结果显示M-SPAHL的fimA与尼泊尔M的fimA不同。M-SPAHL的fimA预测氨基酸序列与任何已知血清群或血清型均无同源性。最相似的序列来自血清型F1，而非尼泊尔M。文中讨论了血清学相关性和序列差异的后果。
