Noda H, Koizumi Y, Zhang Q, Deng K
National Institute of Sericultural and Entomological Science, Tsukuba, Ibaraki 305-8634, Japan.
Insect Biochem Mol Biol. 2001 Apr 27;31(6-7):727-37. doi: 10.1016/s0965-1748(00)00180-6.
Wolbachia, a bacterial endosymbiote of arthropods, causes cytoplasmic incompatibility (CI) in many insect species. CI traits were studied in two planthopper species, Laodelphax striatellus and Sogatella furcifera, and Wolbachia densities in these planthopper species were calculated by quantitative PCR methods. The CI level of L. striatellus was quite high and even aged males strongly caused CI. In contrast, S. furcifera showed partial CI, and males lost their ability to cause CI with age. Wolbachia infecting these two planthopper species were the same with respect to the nucleotide sequences of Wolbachia genes, 16S rDNA, ftsZ gene, groE genes, and wsp gene. Two methods for quantitative PCR, one using a DNA sequencer and the other a real-time sequence detection system, were established to calculate the amount of Wolbachia in the planthoppers. The density of Wolbachia in S. furcifera males was quite low. The difference in CI levels between the two planthopper species seems to be due to different amounts of Wolbachia infecting males.
沃尔巴克氏体是节肢动物的一种胞内共生细菌，在许多昆虫物种中会引发细胞质不亲和（CI）现象。我们对两种飞虱，即灰飞虱和白背飞虱的CI特性进行了研究，并通过定量PCR方法计算了这些飞虱物种体内的沃尔巴克氏体密度。灰飞虱的CI水平相当高，即使是老龄雄虫也会强烈引发CI。相比之下，白背飞虱表现出部分CI现象，且雄虫随着年龄增长会失去引发CI的能力。感染这两种飞虱的沃尔巴克氏体在沃尔巴克氏体基因、16S rDNA、ftsZ基因、groE基因和wsp基因的核苷酸序列方面是相同的。我们建立了两种定量PCR方法，一种使用DNA测序仪，另一种使用实时序列检测系统，以计算飞虱体内沃尔巴克氏体的数量。白背飞虱雄虫体内的沃尔巴克氏体密度相当低。这两种飞虱物种在CI水平上的差异似乎是由于感染雄虫的沃尔巴克氏体数量不同所致。
