Hill T W
J Bacteriol. 1975 May;122(2):743-8. doi: 10.1128/jb.122.2.743-748.1975.
Ascosporogenesis in Nannizzia gypsea was studied by electron microscopy. Development of ascospores began with the formation of an ascus vesicle composed of two paired unit membranes. Myelin figures consisting of coiled or concentric membranes were regularly connected with the growing ascus vesicle. Both the ascus vesicle and the myelin figures possessed an electron-dense line between paired membranes, and both were stained by the periodic acid-silver methenamine technique. Invagination of the ascus vesicle about the haploid nuclei resulted in eight uninucleate prospores bounded by two concentric membranes. Spore wall material was deposited between the two membranes of the prospores, and deposition was greatest in areas of the wall overlying stacked elements of endoplasmic reticulum. A single myelin figure surrounded by a polysaccharide halo was observed in the spore.
利用电子显微镜对石膏样奈瑟菌的子囊孢子形成过程进行了研究。子囊孢子的发育始于由两个成对的单位膜组成的子囊泡的形成。由盘绕或同心膜组成的髓鞘样结构与生长中的子囊泡有规律地相连。子囊泡和髓鞘样结构在成对的膜之间都有一条电子致密线，并且两者都能用高碘酸-硝酸银六胺技术染色。子囊泡围绕单倍体细胞核内陷，形成八个由两个同心膜包围的单核前孢子。孢子壁物质沉积在前孢子的两层膜之间，在内质网堆叠元件上方的壁区域沉积最多。在孢子中观察到一个被多糖晕包围的单个髓鞘样结构。
