Sandoval H, Manga-González Y, Campo R, García P, Castro J M, Pérez de la Vega M
Departamento de Sistemas de Producción y Sanidad Animal, Estación Agrícola Experimental, Consejo Superior de Investigaciones Científicas, León, Spain.
Parasitol Int. 1999 Mar;48(1):21-6. doi: 10.1016/s1383-5769(98)00035-x.
Genetic variability of adult specimens of Dicrocoelium dendriticum has been studied using random amplified polymorphic DNA (RAPD). The worms were collected from the infected livers of different sheep from several localities in León province (NW Spain). DNA fragments were amplified by means of decamer primer oligonucleotides of arbitrary sequence. Some primers produce complex and highly variable patterns of amplified DNA in D. dendriticum. Intra- and inter-population genetic variability of adult parasites were analyzed, scoring polymorphic and monomorphic reproducible bands by means of the Jaccard similarity, and dendrograms showing genetic relationships between individuals were obtained using the FITCH method. Genetic variability seems to be high in this parasite and genetic similarity within a population (worms infecting a single animal) is similar to the average similarity between worms from different sheep. These results suggest that each sheep is infected by numerous genetically different parasites from one or more populations of infected ants.
利用随机扩增多态性DNA（RAPD）技术对枝双腔吸虫成虫样本的遗传变异性进行了研究。这些蠕虫采自西班牙西北部莱昂省几个地区不同绵羊的感染肝脏。通过任意序列的十聚体引物寡核苷酸扩增DNA片段。一些引物在枝双腔吸虫中产生复杂且高度可变的扩增DNA模式。分析了成年寄生虫的种群内和种群间遗传变异性，通过杰卡德相似度对多态性和单态性可重复条带进行评分，并使用菲奇方法获得显示个体间遗传关系的树状图。这种寄生虫的遗传变异性似乎很高，一个种群内（感染单只动物的蠕虫）的遗传相似性与来自不同绵羊的蠕虫之间的平均相似性相似。这些结果表明，每只绵羊都受到来自一个或多个感染蚂蚁种群的众多遗传上不同的寄生虫的感染。
