来自菲律宾株日本血吸虫的编码主要内质网相关钙结合蛋白钙网蛋白的cDNA的分子克隆及功能表达

Molecular cloning and functional expression of a cDNA encoding the major endoplasmic reticulum-associated calcium-binding protein, calreticulin, from Philippine strain Schistosoma japonicum.

作者信息

Scott J C, McManus D P

机构信息

Molecular Parasitology Unit, Australian Centre for International and Tropical Health and Nutrition, The University of Queensland and The Queensland Institute of Medical Research, Australia.

出版信息

Parasitol Int. 1999 Mar;48(1):35-46. doi: 10.1016/s1383-5769(98)00039-7.

DOI:10.1016/s1383-5769(98)00039-7

PMID:11269324

Abstract

We describe the cloning of a full length calreticulin (CR)-encoding cDNA clone isolated by immunoscreening of a cDNA library prepared with mRNA from adult worms of the Philippine strain of Schistosoma japonicum, the cause of Asian schistosomiasis. The sequence of the cDNA is presented, and its molecular characterisation and functional expression as a Ca2+-binding protein described. The potential role of CR in inducing protective immunity in the schistosomes is discussed.

摘要

我们描述了通过对日本血吸虫菲律宾株成虫mRNA制备的cDNA文库进行免疫筛选而分离出的一个全长钙网蛋白（CR）编码cDNA克隆。给出了该cDNA的序列，并描述了其作为一种钙结合蛋白的分子特征和功能表达。讨论了CR在诱导血吸虫保护性免疫中的潜在作用。