Lewis A V, James T J, McGuire J B, Taylor R P
Department of Clinical Biochemistry, Oxford Radcliffe Hospital, Headington, UK.
Ann Clin Biochem. 2001 Mar;38(Pt 2):111-4. doi: 10.1258/0004563011900399.
An immunoturbidimetric assay for cystatin C was optimized with respect to assay imprecision. After investigating the optimum pH, polyethylene glycol concentration and specimen volume, two modifications were introduced: an increase in specimen volume to 25 microL; and an extension of the pre-incubation period to 240 s. These modifications produced an assay with between-batch imprecision (coefficient of variation, n = 10 or 11) ranging from 3-9% at 0.72 mg/L to 1.3% at 5.29 mg/L. The assay was susceptible to interference from lipaemia and haemolysis but not bilirubinaemia in both the original and modified protocol. Extending the pre-incubation to 240 s improved tolerance to common interferences and retained assay applicability in the routine clinical setting.
针对胱抑素C的免疫比浊法在检测不精密度方面进行了优化。在研究了最佳pH值、聚乙二醇浓度和样本体积后，引入了两项改进措施：将样本体积增加到25微升；将预孵育时间延长至240秒。这些改进使该检测方法的批间不精密度（变异系数，n = 10或11）在0.72毫克/升时为3%-9%，在5.29毫克/升时为1.3%。在原始方案和改进方案中，该检测方法均易受脂血和溶血的干扰，但不受黄疸的干扰。将预孵育时间延长至240秒提高了对常见干扰的耐受性，并在常规临床环境中保留了检测方法的适用性。
