Commo S, Gaillard O, Bernard B A
L'Oréal-Centre de recherche C. Zviak, Groupe biologie du cheveu, 90 rue du général Roguet 92583 Clichy, France.
Differentiation. 2000 Dec;66(4-5):157-64. doi: 10.1046/j.1432-0436.2000.660401.x.
Up to now, the localization of stem cells in human anagen hair follicle relied on three complementary approaches; namely, detection of slow cycling cells, detection of high colony forming cells, and differential immunohistochemical staining. These techniques, however, gave conflicting results since stem cells were localized either as long label retaining cells in the so-called bulge area or as high colony forming cells in the lower third of the follicle. In the present study we investigated the expression of cytokeratin 19, a marker for putative stem cell-containing epithelial compartments, in order to characterize stem cell distribution in the human hair follicle throughout the hair cycle. We found that anagen human hair follicles contain two distinct reservoirs for stem cells located in the upper and lower thirds of the follicle. These two reservoirs fuse during the catagentelogen transition phase and individualize again in the newly forming anagen hair follicle.
到目前为止，人类生长期毛囊中干细胞的定位依赖于三种互补的方法；即，慢循环细胞的检测、高集落形成细胞的检测以及差异免疫组织化学染色。然而，这些技术给出了相互矛盾的结果，因为干细胞要么定位为所谓隆突区的长期标记保留细胞，要么定位为毛囊下三分之一处的高集落形成细胞。在本研究中，我们研究了细胞角蛋白19的表达，细胞角蛋白19是假定含有干细胞的上皮区室的标志物，以便在整个毛发周期中表征人类毛囊中干细胞的分布。我们发现，生长期人类毛囊在毛囊的上三分之一和下三分之一处含有两个不同的干细胞储存库。这两个储存库在退行期-静止期过渡阶段融合，并在新形成的生长期毛囊中再次分离。
