[慢性浆液性中耳炎患儿呼吸道合胞病毒和腺病毒的鉴定]
[Identification of respiratory syncytial virus and adenovirus in children with chronic serous otitis].
作者信息
Maseda E, Llorente J L, Melón S, Oña M, Moro M, Manrique C, Suarez C
机构信息
Departamento de ORL, Hospital Central de Asturias, Celestino Villamil s/n. 33006 Oviedo.
出版信息
Acta Otorrinolaringol Esp. 2000 Nov-Dec;51(8):687-90.
OBJECTIVE
To study the presence of respiratory syncytial virus (RSV) and adenovirus in the effusions of the middle ear and adenoid tissue from patients with chronic otitis media with effusion by use of the polymerase chain reaction.
METHOD
The effusions and adenoid samples were collected from 32 children undergoing myringotomy and ventilation tube placement for chronic otitis media with effusion. The samples were separated for viral culture, immunofluorescence and PCR analysis.
RESULTS
One (3%) effusion sample was positive for adenovirus by the PCR. None of samples (effusion and adenoid) were positives for RSV. The results of viral culture and immunofluorescence were all negative.
CONCLUSIONS
Our results can not support that respiratory viruses play a role in the pathogenesis of chronic otitis media with effusion.
目的
通过聚合酶链反应研究慢性分泌性中耳炎患者中耳积液及腺样体组织中呼吸道合胞病毒（RSV）和腺病毒的存在情况。
方法
收集32例因慢性分泌性中耳炎行鼓膜切开置管术的儿童的中耳积液和腺样体样本。将样本分别进行病毒培养、免疫荧光和PCR分析。
结果
1份（3%）中耳积液样本经PCR检测腺病毒呈阳性。所有样本（中耳积液和腺样体）呼吸道合胞病毒均为阴性。病毒培养和免疫荧光结果均为阴性。
结论
我们的结果不支持呼吸道病毒在慢性分泌性中耳炎发病机制中起作用。
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