Diaz S L, Kemmling A K, Rubio M C, Balerio G N
Cátedra de Farmacología, Facultad de Farmacia y Bioquímica (Universidad de Buenos Aires), Argentina.
Behav Pharmacol. 2001 Feb;12(1):75-9. doi: 10.1097/00008877-200102000-00009.
In previous studies we have demonstrated a possible interaction between the GABAergic and opioid systems involved in the antinociceptive effect of the GABA(B) agonist, baclofen (BAC). On the other hand, sex differences have been observed for the antinociceptive effect of morphine (MOR). In the present study, we analyzed sex-related differences in the MOR abstinence syndrome and its prevention with BAC. Prepubertal male and female Swiss-Webster albino mice (27-33 g) were rendered dependent by intraperitoneal (i.p.) injection of MOR (2, 4 and 8 mg/kg), twice daily for 9 days. On the tenth day the dependent animals were divided into two groups: one received naloxone (NAL) (6 mg/kg, i.p.) 60 min after the last dose of MOR, to precipitate the abstinence syndrome; the other group received BAC (2 mg/kg, i.p.) followed by NAL (6 mg/kg, i.p.), injected 30 and 60 min after the last dose of MOR, respectively. Behavioral signs were recorded in the open field for 30 min. Although there were sex differences in the MOR withdrawal syndrome, we found a lack of sex differences in the prevention of the MOR abstinence syndrome by BAC.
在先前的研究中，我们已经证明参与γ-氨基丁酸B（GABA(B)）激动剂巴氯芬（BAC）镇痛作用的GABA能系统和阿片系统之间可能存在相互作用。另一方面，已经观察到吗啡（MOR）的镇痛作用存在性别差异。在本研究中，我们分析了MOR戒断综合征中的性别相关差异以及BAC对其的预防作用。对青春期前的雄性和雌性瑞士韦伯斯特白化小鼠（体重27 - 33克）通过腹腔注射MOR（2、4和8毫克/千克）使其产生依赖性，每天两次，持续9天。在第10天，将依赖动物分为两组：一组在最后一剂MOR后60分钟接受纳洛酮（NAL）（6毫克/千克，腹腔注射），以引发戒断综合征；另一组接受BAC（2毫克/千克，腹腔注射），然后分别在最后一剂MOR后30分钟和60分钟接受NAL（6毫克/千克，腹腔注射）。在旷场中记录行为体征30分钟。虽然MOR戒断综合征存在性别差异，但我们发现BAC预防MOR戒断综合征不存在性别差异。
