Sippell G, Lehmann P, Hollmann G
J Chromatogr. 1975 May 21;108(2):305-12. doi: 10.1016/s0021-9673(00)84673-7.
An automated method of Sephacex LH-20 chromatography has been developed for the simultaneous and complete separation of corticosterone, 11-deoxycortisol, aldosterone, cortisone and cortisol from a small plasma sample prior to radioimmunoassay. Using a controlled elution flow-rate of 40 ml/h and automated programme tape-controlled collection of the eluates from six columns eluted simultaneously, excellent reproducibility of elution patterns with minimal day-to-day and column-to-column variations could be achieved. The method can be easily extended and permits the reliable, rapid and easy routine separation of individual steroids from a single plasma or tissue extract on a multi-column scale.
已开发出一种自动的Sephacex LH - 20色谱法，用于在放射免疫测定之前从小份血浆样品中同时且完全地分离皮质酮、11 - 脱氧皮质醇、醛固酮、可的松和皮质醇。使用40毫升/小时的受控洗脱流速，并通过自动程序带控制同时洗脱的六根柱的洗脱液收集，可实现洗脱模式的出色重现性，日常和柱间变化最小。该方法可轻松扩展，并允许在多柱规模上从单个血浆或组织提取物中可靠、快速且容易地常规分离各个类固醇。
