培哚普利、普萘洛尔和双氢氯噻嗪对自发性高血压大鼠心血管重塑的影响。

Effects of perindopril, propranolol, and dihydrochlorothiazide on cardiovascular remodelling in spontaneously hypertensive rats.

作者信息

Su J Z, Chen S C, Wu K G, Chen D G, Rui H B, Wang X Y, Wang H J

机构信息

Department of Cardiology, First Affiliated Hospital of Fujian Medical University, Fujian Institute of Hypertension Research, Fuzhou 350005, China.

出版信息

Zhongguo Yao Li Xue Bao. 1999 Oct;20(10):923-8.

PMID:11270993

Abstract

AIM

To investigate the effects of perindopril, propranolol, and dihydrochlorothiazide on artery wall thickening, left ventricular hypertrophy, and cardiac fibrosis in spontaneously hypertensive rats (SHR).

METHODS

After measurement of systolic blood pressure (SBP), 16-wk-old Male SHR were randomly divided into 3 groups (each n = 10), given perindopril (Per, 5 mg.kg-1.d-1), propranolol (Pro, 40 mg.kg-1.d-1), dihydrochlorothiazide (DCT, 100 mg.kg-1.d-1) respectively by gavage for 12 wk. Sex-, age-, and number-matched untreated SHR and normotensive Wistar Kyoto rats (WKY) served as controls. When the treatment finished, body weights (BW) and SBP were measured before decapitation of the rats. The heart was excised rapidly, the left ventricle was weighed and then subjected to collagen content analysis. Vascular wall and lumen ratio from aorta, renal arteries and branch III vessels of mesenteric arteries were determined morphometrically.

RESULTS

Treated rats in 3 groups showed a lower SBP and the ratio of left ventricle weight to body weight (LVW/BW) compared with WKY. Artery wall thickening was similarly inhibited in the treated groups. Per and Pro inhibited cardiac fibrosis, but collagen concentration increased in DCT treated SHR [collagen volume fraction (CVF): 19 +/- 4 vs SHR 14 +/- 4, P < 0.05; perivascular collagen fraction(PVCF): 84 +/- 7 vs SHR 79 +/- 5, P < 0.05].

CONCLUSION

Per and Pro inhibited, but DCT promoted, cardiac fibrosis.

摘要

目的

研究培哚普利、普萘洛尔和双氢氯噻嗪对自发性高血压大鼠（SHR）动脉壁增厚、左心室肥厚和心脏纤维化的影响。

方法

测量收缩压（SBP）后，将16周龄雄性SHR随机分为3组（每组n = 10），分别给予培哚普利（Per，5 mg·kg-1·d-1）、普萘洛尔（Pro，40 mg·kg-1·d-1）、双氢氯噻嗪（DCT，100 mg·kg-1·d-1）灌胃12周。将性别、年龄和数量匹配的未治疗SHR和正常血压的Wistar Kyoto大鼠（WKY）作为对照。治疗结束后，在大鼠断头前测量体重（BW）和SBP。迅速取出心脏，称量左心室重量，然后进行胶原含量分析。用形态计量学方法测定主动脉、肾动脉和肠系膜动脉Ⅲ级分支血管的血管壁与管腔比值。

结果

与WKY相比，3组治疗大鼠的SBP和左心室重量与体重之比（LVW/BW）均较低。治疗组的动脉壁增厚同样受到抑制。Per和Pro抑制心脏纤维化，但DCT治疗的SHR中胶原浓度升高[胶原体积分数（CVF）：19±4 vs SHR 14±4，P < 0.05；血管周围胶原分数（PVCF）：84±7 vs SHR 79±5，P < 0.05]。