Schön M R, Puhl G, Gerlach J, Frank J, Neuhaus P
Department of Surgery, Rudolf Virchow Klinikum, Freie Universität Berlin, Germany.
Transpl Int. 1994;7 Suppl 1:S159-62. doi: 10.1111/j.1432-2277.1994.tb01337.x.
Hepatocyte cultures have been used extensively for a wide variety of physiological, pharmacological and experimental studies. The warm ischaemic period before isolation is kept to a minimum to achieve a high yield of cells isolated and a good viability for culture. We have recently introduced a new concept of liver resuscitation after warm ischaemia that is based on a 3-h reperfusion period with an improved perfusate and simultaneous dialysis. In this study, we applied the new technique for hepatocyte isolation from livers subjected to 80 min of complete ischaemia at 37 degrees C. Cell yield was improved by a resuscitating perfusion from 58% to 73% and viability from 39% to 76%.
肝细胞培养已广泛应用于各种生理学、药理学和实验研究。在分离前将热缺血期保持在最短时间，以获得高产量的分离细胞和良好的培养活力。我们最近引入了热缺血后肝脏复苏的新概念，该概念基于使用改良灌注液并同时进行透析的3小时再灌注期。在本研究中，我们将这项新技术应用于从在37℃下经历80分钟完全缺血的肝脏中分离肝细胞。通过复苏灌注，细胞产量从58%提高到73%，活力从39%提高到76%。
