Ustinov J, Lahtinen T, Bruggeman C, Häyry P, Lautenschlager I
Transplantation Laboratory, University of Helsinki, Finland.
Transpl Int. 1994;7 Suppl 1:S381-2. doi: 10.1111/j.1432-2277.1994.tb01397.x.
Cytomegalovirus (CMV) has been demonstrated to induce class II antigen expression in endothelial cells. To study whether ganciclovir (DHPG) has an effect on CMV-induced class II expression, cultured rat heart endothelial cells were infected with rat CMV (RCMV) and treated with different DHPG concentrations. Class II antigens in endothelial cells were detected by a monoclonal antibody and immunoperoxidase technique. Control cells did not express class II antigen, but during RCMV infection 92% of cells were class II-positive. DHPG treatment (1, 10, 100 and 1000 microg/ml) decreased RCMV-induced class II expression from 73% to 59%, 6% and 0%, respectively. As DHPG inhibits CMV DNA polymerase, our present results suggest that DHPG affects RCMV-induced class II expression via the inhibition of RCMV DNA replication.
巨细胞病毒（CMV）已被证明可在内皮细胞中诱导II类抗原表达。为研究更昔洛韦（DHPG）是否对CMV诱导的II类表达有影响，用大鼠巨细胞病毒（RCMV）感染培养的大鼠心脏内皮细胞，并给予不同浓度的DHPG处理。采用单克隆抗体和免疫过氧化物酶技术检测内皮细胞中的II类抗原。对照细胞不表达II类抗原，但在RCMV感染期间，92%的细胞II类抗原呈阳性。DHPG处理（1、10、100和1000微克/毫升）使RCMV诱导的II类表达分别从73%降至59%、6%和0%。由于DHPG抑制CMV DNA聚合酶，我们目前的结果表明，DHPG通过抑制RCMV DNA复制影响RCMV诱导的II类表达。
