Sunamori M, Miyamoto H, Yoshida T, Suzuki A
Department of Thoracic-Cardiovascular Surgery, Tokyo Medical and Dental University, School of Medicine, Japan.
Transpl Int. 1994;7 Suppl 1:S485-8. doi: 10.1111/j.1432-2277.1994.tb01425.x.
Isolated canine hearts were preserved for 12 h at 5 degrees C followed by normothermic reperfusion for 2 h. Dogs were divided into two groups: group 1 (n = 7) received a nondepolarizing preservation solution in multidose, and group 2 (n = 6) received single-flushed University of Wisconsin (UW) solution, both administered in multidose fashion. At the end of reperfusion, the myocardial adenosine triphosphate concentration and left ventricular systolic and diastolic function were preserved better in groupl than in group 2. Myocardial mitochondrial ultrastructural integrity was identical in the two groups. These results suggested that in a 12-h heart preservation, nondepolarizing solution administered in multidose fashion protects the myocardium from the deleterious effects of hypothermia and cardioplegia better than UW solution.
将离体犬心在5℃保存12小时，随后进行2小时的常温再灌注。犬被分为两组：第1组（n = 7）接受多剂量非去极化保存液，第2组（n = 6）接受单次冲洗的威斯康星大学（UW）溶液，均采用多剂量方式给药。再灌注结束时，第1组心肌三磷酸腺苷浓度以及左心室收缩和舒张功能的保存情况优于第2组。两组心肌线粒体超微结构完整性相同。这些结果表明，在12小时心脏保存中，多剂量给药的非去极化溶液比UW溶液能更好地保护心肌免受低温和心脏停搏的有害影响。
