Hunt K J, Lemasters J J, Thurman R G
Department of Pharmacology, The University of North Carolina, Chapel Hill 27599-7365, USA.
Transpl Int. 1994;7 Suppl 1:S493-5. doi: 10.1111/j.1432-2277.1994.tb01427.x.
Adenosine is a key component of Carolina rinse solution, which was developed to prevent an O2-dependent reperfusion injury following liver transplantation. Kupffer cells are activated following liver transplantation, an effect minimized by adenosine. In this study, the concentration of adenosine was varied in Carolina rinse, Ringer's, and Ringer's containing hydroxyethyl starch. Following 24 h of cold storage in University of Wisconsin cold storage solution, colloidal carbon uptake was measured to assess phagocytosis by Kupffer cells. Carbon uptake was elevated significantly in this model from values of 136 +/- 15 in unstored control livers to 187 +/- 25 mg/g per hour following a rinse with 10 ml of Ringer's solution. Phagocytosis was reduced about 50% when 0.1 mM adenosine was added to the Ringer's solution; however, addition of up to 1 mM adenosine in Carolina Rinse did not prevent elevated phagocytosis. In contrast, values were reduced significantly by addition of 5-10 mM adenosine to Carolina Rinse or Ringer's containing hydroxyethyl starch. Thus, the higher concentrations of adenosine needed to reduce Kupffer cell activation can best be explained by binding of adenosine to hydroxyethyl starch.
腺苷是卡罗来纳冲洗液的关键成分，该冲洗液旨在预防肝移植后氧依赖性再灌注损伤。肝移植后库普弗细胞被激活，而腺苷可将这种效应降至最低。在本研究中，在卡罗来纳冲洗液、林格氏液和含羟乙基淀粉的林格氏液中改变腺苷浓度。在威斯康星大学冷保存液中冷保存24小时后，测量胶体碳摄取量以评估库普弗细胞的吞噬作用。在该模型中，用10毫升林格氏液冲洗后，碳摄取量从未保存的对照肝脏的136±15显著升高至187±25毫克/克每小时。当向林格氏液中添加0.1毫摩尔腺苷时，吞噬作用降低约50%；然而，在卡罗来纳冲洗液中添加高达1毫摩尔腺苷并不能防止吞噬作用升高。相比之下，向卡罗来纳冲洗液或含羟乙基淀粉的林格氏液中添加5 - 10毫摩尔腺苷可使数值显著降低。因此，降低库普弗细胞激活所需的较高浓度腺苷，最好用腺苷与羟乙基淀粉的结合来解释。
