Tkáciková L, Kantíková M, Dmitriev A, Mikula I
University of Veterinary Medicine, 041 81 Kosice, Slovakia.
Folia Microbiol (Praha). 2000;45(2):157-60. doi: 10.1007/BF02817416.
Nineteen serogroup 1/2a Listeria monocytogenes strains isolated from raw milk, dairy products and salt water in one dairy were analyzed. Pulsed field gel electrophoresis (PFGE) and ribotyping were used to determine whether these strains isolated over a 8-month period are epidemiologically related. The samples of raw milk were contaminated by different L. monocytogenes clones. The clones isolated from dairy products (with the exception of one sample) and salt water were identical. Comparative genetic analysis of the clones isolated from raw milk, salt water and dairy products revealed the source of contamination and identified the L. monocytogenes strain involved in this process.
对从一家乳制品厂的生牛奶、乳制品和盐水中分离出的19株血清型1/2a单核细胞增生李斯特菌菌株进行了分析。采用脉冲场凝胶电泳（PFGE）和核糖体分型法来确定在8个月期间分离出的这些菌株在流行病学上是否相关。生牛奶样本被不同的单核细胞增生李斯特菌克隆污染。从乳制品（除一个样本外）和盐水中分离出的克隆是相同的。对从生牛奶、盐水和乳制品中分离出的克隆进行比较遗传分析，揭示了污染来源，并确定了参与这一过程的单核细胞增生李斯特菌菌株。
