Boerlin P, Bannerman E, Ischer F, Rocourt J, Bille J
Swiss National Centre for Listeriosis, Institute for Microbiology, CHUV, Lausanne.
Res Microbiol. 1995 Jan;146(1):35-49. doi: 10.1016/0923-2508(96)80269-5.
One hundred Listeria monocytogenes strains were typed by random amplification of polymorphic DNA (RAPD) with three different primers, and the results were compared with those obtained by serotyping, ribotyping, multilocus enzyme electrophoresis, restriction enzyme analysis and phage typing. The RAPD patterns of strains appear to be stable during epidemics even over periods of several years. Reproducibility of the RAPD patterns was good. The discriminatory power of RAPD typing was the best among all the methods tested. RAPD is therefore a very promising tool in the study of listeriosis epidemiology. However, the problems related to the standardization of the technique first have to be resolved before the wide use of RAPD is possible.
采用三种不同引物通过随机扩增多态性DNA（RAPD）技术对100株单核细胞增生李斯特菌进行分型，并将结果与血清分型、核糖体分型、多位点酶电泳、限制性酶切分析及噬菌体分型所获结果进行比较。菌株的RAPD图谱在疫情期间似乎是稳定的，即使历经数年。RAPD图谱的可重复性良好。在所有测试方法中，RAPD分型的鉴别能力最佳。因此，RAPD是研究李斯特菌病流行病学的一个非常有前景的工具。然而，在RAPD能够广泛应用之前，首先必须解决与该技术标准化相关的问题。
