George S P, Ahmad A, Rao M B
Division of Biochemical Sciences, National Chemical Laboratory, Pune 411 008, India.
Bioresour Technol. 2001 Apr;77(2):171-5. doi: 10.1016/s0960-8524(00)00150-4.
A novel alkalothermophilic actinomycete having optimum growth at pH 9 and 50 degrees C was isolated from self-heating compost from the Barabanki district of Uttar Pradesh, India. Based on its morphology, susceptibility of spores to heat and novobiocin, guaninecytosine content of chromosomal DNA and cell wall composition, the organism was classified under Thermomonospora. The alkalothermophilic actinomycete produced 23 IU/ml carboxymethyl cellulase (CMCase). The CMCase was purified by fractional ammonium sulphate precipitation followed by cellulose affinity chromatography and Sephacryl S-200 gel filtration. The CMCase had a molecular weight of 38 KD and pI of 4.1. The enzyme exhibited optimum activity at pH 5 and temperature 50 degrees C. The CMCase showed pH stability in the range 7-10. The enzyme retained 100% activity at 50 degrees C for 72 h and had half-lives of 7 and 3 h at 60 degrees C and 70 degrees C, respectively. The CMCase was stable in the presence of commercial detergents such as Ariel, Henko and Surf Excel, indicating its potential as an additive to laundry detergents.
从印度北方邦巴拉班基区的自热堆肥中分离出一种新型嗜碱嗜热放线菌，其在pH 9和50℃条件下生长最佳。基于其形态、孢子对热和新生霉素的敏感性、染色体DNA的鸟嘌呤 - 胞嘧啶含量以及细胞壁组成，该微生物被归类为嗜热单孢菌属。这种嗜碱嗜热放线菌产生23 IU/ml的羧甲基纤维素酶（CMCase）。通过分级硫酸铵沉淀，随后进行纤维素亲和层析和Sephacryl S - 200凝胶过滤对CMCase进行纯化。CMCase的分子量为38 KD，pI为4.1。该酶在pH 5和温度50℃时表现出最佳活性。CMCase在pH 7 - 10范围内表现出pH稳定性。该酶在50℃下72小时保持100%活性，在60℃和70℃时的半衰期分别为7小时和3小时。CMCase在诸如碧浪、汉高和汰渍等市售洗涤剂存在下稳定，表明其作为洗衣洗涤剂添加剂的潜力。
