Bach M K, Brashler J R
J Immunol. 1975 Jun;114(6):1799-807.
DBA/1 mice were immunized with low doses of ovalbumin and alumina gel to elicit an IgE response which could be augmented upon reimmunization. Treatment of IgE-producing mice with high doses of chemically modified ovalbumin preparations markedly reduced or eliminated the ability of the mice to respond to subsequent reimmunizations with low doses of ovalbumin. It was found that the primary structural requirement for efficacy of the modified ovalbumins was reduced antigenicity to prevent the anaphylactic demise of the animals. Cumulative doses of 0.5 mg or more given in several increments, i.v., afforded optimal protection. The protection was antigen-specific and persisted for 2 to 3 months regardless of intervening exposures of the animals to low doses of antigen. It could be extended by further courses of treatment. There was no correlation between the levels of circulating hemagglutinating or total antibody and the induction of unresponsiveness. Nor was the passive administration of antibody able to approximate the effects of the modified antigens. It is suggested that the induction of the IgE class specific unresponsiveness in responding animals may take p;ace via effects on T cells.
用低剂量的卵清蛋白和氧化铝凝胶对DBA/1小鼠进行免疫，以引发IgE反应，再次免疫时该反应会增强。用高剂量化学修饰的卵清蛋白制剂处理产生IgE的小鼠，可显著降低或消除小鼠对随后低剂量卵清蛋白再次免疫的反应能力。研究发现，修饰后的卵清蛋白发挥功效的主要结构要求是降低抗原性，以防止动物因过敏反应而死亡。静脉内分几次给予累积剂量达0.5毫克或更多，可提供最佳保护。这种保护具有抗原特异性，并且持续2至3个月，无论动物期间是否接触低剂量抗原。通过进一步的治疗疗程可延长保护时间。循环血凝素或总抗体水平与无反应性的诱导之间没有相关性。被动给予抗体也无法模拟修饰抗原的效果。有人提出，在反应动物中诱导IgE类特异性无反应性可能是通过对T细胞的作用实现的。
