Durbec P, Rougon G
Laboratoire de Génétique et Physiologie du Développement, IBDM, CNRS/INSERM/Université de la Méditérranée/AP de Marseille, Parc Scientifique de Luminy, Marseille Cedex 9, 13288, France.
Mol Cell Neurosci. 2001 Mar;17(3):561-76. doi: 10.1006/mcne.2000.0951.
In vertebrates, interneurons of the olfactory bulb are continuously generated postnatally and throughout life at the subventricular zone of the forebrain. From there, the neuronal progenitors migrate tangentially in a typical chain-like structure to the olfactory bulb in which they differentiate as interneurons. We have used a mouse/chick xenograft strategy to explore the migration and differentiation potential of the mouse olfactory progenitors in a heterochronic and heterotypic environment. We compared the migration of primary cells derived from the subventricular zone of adult or newborn lateral ventricule with the behavior of in vitro amplified cells derived from the same structures. We show that in the chick environment, olfactory bulb progenitors from newborn brain tissue perform chain migration along the neural crest cell routes, whereas grafted neurosphere-derived-cells migrate as isolated cells. These results, together with in vitro observations, allow us to propose that neuronal chain migration is a community effect independent of environmental cues but which is closely regulated by the differentiation program of the cells. We established that the progenitor cells performing chain migration are already committed, while neurosphere-derived-cells are able to integrate and differentiate as components of the peripheral nervous system.
在脊椎动物中，嗅球中间神经元在出生后及整个生命过程中持续在前脑的脑室下区产生。从那里，神经祖细胞以典型的链状结构沿切线方向迁移至嗅球，并在其中分化为中间神经元。我们采用小鼠/鸡异种移植策略，在异时性和异型环境中探究小鼠嗅觉祖细胞的迁移和分化潜能。我们比较了源自成年或新生侧脑室脑室下区的原代细胞的迁移情况，以及源自相同结构的体外扩增细胞的行为。我们发现，在鸡的环境中，来自新生脑组织的嗅球祖细胞沿神经嵴细胞路径进行链式迁移，而移植的神经球衍生细胞则以单个细胞的形式迁移。这些结果，连同体外观察结果，使我们提出神经元链式迁移是一种独立于环境线索的群体效应，但受到细胞分化程序的密切调控。我们确定，进行链式迁移的祖细胞已经定向分化，而神经球衍生细胞能够作为周围神经系统的组成部分整合并分化。
