Riek R
Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule Hönggerberg, Zürich, CH-8093, Switzerland.
J Magn Reson. 2001 Mar;149(1):149-53. doi: 10.1006/jmre.2001.2291.
Hydrogen bond lengths in Watson-Crick base pairs can be characterized by cross-correlated relaxation between 1H chemical shift anisotropy and dipole-dipole coupling of 1H and its hydrogen bond acceptor 15N. As a reference, the cross-correlated relaxation between 1H chemical shift anisotropy and dipole-dipole coupling of 1H and its hydrogen bond donor 15N is used. With the two measured cross-correlated relaxation rates, an apparent hydrogen bond length can be determined, which is composed by the hydrogen bond length multiplied by a term representing the amplitude of inter-base motions. Data are presented for the 15N3-1H3...15N1 hydrogen bonds in A=T base pairs of the Antennapedia homeodomain-DNA complex with a correlation time of global rotational diffusion of 20 ns.
沃森-克里克碱基对中的氢键长度可通过1H化学位移各向异性与1H及其氢键受体15N的偶极-偶极耦合之间的交叉相关弛豫来表征。作为参考，使用1H化学位移各向异性与1H及其氢键供体15N的偶极-偶极耦合之间的交叉相关弛豫。利用所测得的两个交叉相关弛豫率，可确定一个表观氢键长度，该长度由氢键长度乘以一个代表碱基间运动幅度的项组成。文中给出了触角足同源域-DNA复合物中A=T碱基对的15N3-1H3...15N1氢键的数据，其整体旋转扩散的相关时间为20纳秒。
