Pfeifer A, Kessler T, Yang M, Baranov E, Kootstra N, Cheresh D A, Hoffman R M, Verma I M
The Salk Institute, La Jolla, California 92037, USA.
Mol Ther. 2001 Mar;3(3):319-22. doi: 10.1006/mthe.2001.0276.
Viral vectors based on lentiviruses, such as the human immunodeficiency virus, are able to transduce a broad spectrum of nondividing cells in vivo. This ability of lentiviral vectors makes them an attractive vehicle for gene transfer into the liver. In order to determine the requirements for efficient lentiviral gene transfer, we used a fluorescence imaging system, which allows the detection of cells and tissues that express fluorescent reporter genes (e.g., green fluorescence protein) in the living animal. We show that the latest generation of lentiviral vectors efficiently transduces the murine liver. Further analysis demonstrated that neither cell-cycle activation nor division of liver cells is a prerequisite for lentiviral gene transfer in vivo.
基于慢病毒（如人类免疫缺陷病毒）的病毒载体能够在体内转导多种非分裂细胞。慢病毒载体的这种能力使其成为将基因导入肝脏的有吸引力的载体。为了确定高效慢病毒基因转移的要求，我们使用了一种荧光成像系统，该系统能够检测活体动物中表达荧光报告基因（如绿色荧光蛋白）的细胞和组织。我们发现最新一代的慢病毒载体能有效地转导小鼠肝脏。进一步分析表明，细胞周期激活或肝细胞分裂都不是体内慢病毒基因转移的先决条件。
