Copin M C, Buisine M P, Leteurtre E, Marquette C H, Porte H, Aubert J P, Gosselin B, Porchet N
Service d'Anatomie et Cytologie Pathologiques, Hôpital A. Calmette, Lille, France.
Hum Pathol. 2001 Mar;32(3):274-81. doi: 10.1053/hupa.2001.22752.
Lung adenocarcinomas are heterogeneous clinically and histologically. Expression of the mucin genes was analyzed as a molecular marker of glandular cytodifferentiation in primary lung adenocarcinomas. Expression was correlated with histopathologic subtypes of World Health Organization classification with the aim of investigating the histogenesis of primary lung adenocarcinomas. Thirty-four primary lung adenocarcinomas were examined by in situ hybridization for mucin gene expression (MUC1-4, MUC5AC, MUC5B, MUC6-7) and by immunohistochemistry for MUC5AC and MUC5B apomucin expression. Mucinous bronchioloalveolar carcinoma (BAC) had a homogeneous pattern of mucin gene expression different from those of other types of lung adenocarcinoma, involving secreted mucins (MUC5AC, MUC5B, and MUC6) and membrane-bound mucins (MUC1, MUC3, and MUC4). Non-BAC adenocarcinoma and mucinous BAC aberrantly expressed mucin genes MUC3, and MUC3 and MUC6, respectively, which are undetectable in normal fetal and adult lung. Our results show the particular phenotype of mucin gene expression in mucinous type of BACs and the heterogeneous expression of respiratory and nonrespiratory mucins in the other types. This finding supports the theory of a common progenitor cell with the potential of multicellular differentiation. From a practical point of view, the aberrant expression of MUC3 and MUC6 could serve as a diagnostic marker in the management of the mucinous type of bronchioloalveolar carcinomas. HUM PATHOL 32:274-281.
肺腺癌在临床和组织学上具有异质性。对黏蛋白基因的表达进行分析，作为原发性肺腺癌腺细胞分化的分子标志物。将该表达与世界卫生组织分类的组织病理学亚型相关联，目的是研究原发性肺腺癌的组织发生。通过原位杂交检测34例原发性肺腺癌的黏蛋白基因表达（MUC1 - 4、MUC5AC、MUC5B、MUC6 - 7），并通过免疫组织化学检测MUC5AC和MUC5B脱辅基黏蛋白的表达。黏液性细支气管肺泡癌（BAC）具有与其他类型肺腺癌不同的黏蛋白基因表达均一模式，涉及分泌性黏蛋白（MUC5AC、MUC5B和MUC6）和膜结合黏蛋白（MUC1、MUC3和MUC4）。非BAC腺癌和黏液性BAC分别异常表达黏蛋白基因MUC3以及MUC3和MUC6，而在正常胎儿和成人肺中无法检测到这些基因。我们的结果显示了黏液性BAC中黏蛋白基因表达的特殊表型以及其他类型中呼吸性和非呼吸性黏蛋白的异质性表达。这一发现支持了具有多细胞分化潜能的共同祖细胞理论。从实际角度来看，MUC3和MUC6的异常表达可作为黏液性细支气管肺泡癌管理中的诊断标志物。《人类病理学》32:274 - 281。
