Shimonishi T, Miyazaki K, Kono N, Sabit H, Tuneyama K, Harada K, Hirabayashi J, Kasai K, Nakanuma Y
Department of Pathology II, Kanazawa University School of Medicine, Kanazawa, Japan.
Hum Pathol. 2001 Mar;32(3):302-10. doi: 10.1053/hupa.2001.22767.
Galectins, a family of beta-galactoside-binding animal lectins, might be involved in tumor progression. In this study, the expression patterns of galectin-1 and -3 were examined immunohistochemically in intrahepatic cholangiocarcinoma (ICC), with emphasis on its development and progression as well as its histopathologic features, by use of samples of normal intrahepatic bile duct (n = 20), biliary epithelial dysplasia (n = 15), ICC (n = 40), and a cholangiocarcinoma cell line, CCKS1. In normal intrahepatic bile ducts, galectin-3 was constitutively but weakly expressed, whereas galectin-1 was not expressed. In hepatolithiasis, biliary epithelial dysplasia was strongly positive for galectin-3 but negative for galectin-1. Galectin-3 was frequently and strongly expressed in the cytoplasm of well-differentiated ICCs, and its expression was significantly decreased and less intense or even absent in poorly differentiated ICCs. Galectin-1 was expressed in carcinoma cells in ICC, and its incidence and extent were correlated with histologic dedifferentiation of ICC. Proliferative cell nuclear antigen (PCNA) labeling index (LI) was higher in ICC cases positive for galectin-1 than in those that were negative. Galectin-1 was strongly expressed in cancerous stroma of ICC, and this stromal expression was related to histologic dedifferentiation of ICC. In the carcinoma cell line CCKS1, galectin-1 and -3 were expressed in the cytoplasm of carcinoma cells, and galectin-1 was additionally detected in the culture medium. These results suggest that galectin-1 was newly expressed on carcinoma cells of ICC, and its overexpression seems to be associated with neoplastic progression and proliferative activities, and the expression of galectin-1 in cancerous stroma may also be related to the progression of ICC. Galectin-3 expression in epithelial cells is up-regulated in the preneoplastic and early neoplastic stages of ICC, although galectin-3 tends to disappear at later stages of ICC. HUM PATHOL 32:302-310.
半乳糖凝集素是一类结合β-半乳糖苷的动物凝集素，可能参与肿瘤进展。在本研究中，通过使用正常肝内胆管（n = 20）、胆管上皮发育异常（n = 15）、肝内胆管癌（ICC，n = 40）样本以及胆管癌细胞系CCKS1，采用免疫组织化学方法检测了半乳糖凝集素-1和-3在肝内胆管癌中的表达模式，重点关注其发生发展、病理特征。在正常肝内胆管中，半乳糖凝集素-3呈组成性低表达，而半乳糖凝集素-1不表达。在肝内胆管结石症中，胆管上皮发育异常对半乳糖凝集素-3呈强阳性反应，而对半乳糖凝集素-1呈阴性反应。半乳糖凝集素-3在高分化ICC的细胞质中频繁且强表达，而在低分化ICC中其表达显著降低、强度减弱甚至缺失。半乳糖凝集素-1在ICC的癌细胞中表达，其发生率和表达程度与ICC的组织学去分化相关。增殖细胞核抗原（PCNA）标记指数（LI）在半乳糖凝集素-1阳性的ICC病例中高于阴性病例。半乳糖凝集素-1在ICC的癌间质中强表达，且这种间质表达与ICC的组织学去分化相关。在癌细胞系CCKS1中，半乳糖凝集素-1和-3在癌细胞的细胞质中表达，并且在培养基中还检测到半乳糖凝集素-1。这些结果表明，半乳糖凝集素-1在ICC的癌细胞上新表达，其过表达似乎与肿瘤进展和增殖活性相关，并且半乳糖凝集素-1在癌间质中的表达也可能与ICC的进展有关。在ICC的癌前和肿瘤早期阶段，上皮细胞中的半乳糖凝集素-3表达上调，尽管在ICC的后期阶段半乳糖凝集素-3趋于消失。《人类病理学》32:302 - 310。
