Altiokka G, Atkosar Z, Sener E, Tunçel M
Department of Analytical Chemistry, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey.
J Pharm Biomed Anal. 2001 May;25(2):339-42. doi: 10.1016/s0731-7085(00)00509-4.
A flow injection analysis (FIA) of sildenafil citrate (SLD) using UV detection is described in this study. The best solvent system was found to be consisting of 0.2 M phosphate buffer at pH 8 having 10% MeOH. A flow rate of 1 ml. min(-1) was pumped and active material was detected at 292 nm. The calibration equation was linear in the range of 1x10(-6)-5x10(-6) M. Limit of detection (LOD) and limit of quantitation (LOQ) were calculated to be 3x10(-7) and 8.9x10(-7) M with a R.S.D. 1.9 and 0.6% (n=7), respectively. The proposed method was applied to the determination of SLD in VIAGRA tablet, containing 50 mg active material. The results were compared with those obtained from UV-Spectrophotometry. The results showed that there is a good agreement between FIA method and the UV-Spectrophotometry. The validation studies were realised by the related applications and the results were evaluated statistically. According to the results, insignificant difference was observed between the methods.
本研究描述了一种使用紫外检测的枸橼酸西地那非（SLD）流动注射分析（FIA）方法。发现最佳溶剂体系为由pH 8的0.2 M磷酸盐缓冲液和10%甲醇组成。以1 ml·min⁻¹的流速泵送，在292 nm处检测活性物质。校准方程在1×10⁻⁶ - 5×10⁻⁶ M范围内呈线性。检测限（LOD）和定量限（LOQ）分别计算为3×10⁻⁷和8.9×10⁻⁷ M，相对标准偏差（R.S.D.）分别为1.9%和0.6%（n = 7）。所提出的方法应用于含50 mg活性物质的伟哥片中SLD的测定。将结果与紫外分光光度法获得的结果进行比较。结果表明FIA方法与紫外分光光度法之间有良好的一致性。通过相关应用进行验证研究，并对结果进行统计学评估。根据结果，各方法之间未观察到显著差异。
