小鼠中红细胞对骨髓造血干细胞功能活性的调节
Regulation of functional activity of bone marrow hemopoietic stem cells by erythroid cells in mice.
作者信息
Sennikov S V, Inzhelevskaya T V, Eremina L V, Kozlov V A
机构信息
Institute of Clinical Immunology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk.
出版信息
Bull Exp Biol Med. 2000 Dec;130(12):1159-61.
Transplantation of erythroid and bone marrow cells to irradiated mice stimulated exogenous colony formation. Pretreatment of erythroid cells with specific rabbit antiserum to erythroblasts abolished this effect. The reverse transcriptase polymerase chain reaction revealed the presence of mRNA for interleukin-1alpha, interleukin-1beta, interleukin-3, interleukin-6, and granulocyte-macrophage colony-stimulating factor in erythroid cells. Granulocyte-macrophage colony-stimulating factor was found in the conditioned medium from erythroid cells. Thus, erythroid cells stimulated colony-forming activity of bone marrow cells, which was probably mediated via cytokine synthesis (e.g., granulocyte-macrophage colony-stimulating factor).
将红系细胞和骨髓细胞移植到受照射小鼠体内可刺激外源性集落形成。用针对成红细胞的特异性兔抗血清预处理红系细胞可消除这种效应。逆转录聚合酶链反应显示红系细胞中存在白细胞介素-1α、白细胞介素-1β、白细胞介素-3、白细胞介素-6和粒细胞-巨噬细胞集落刺激因子的mRNA。在红系细胞的条件培养基中发现了粒细胞-巨噬细胞集落刺激因子。因此，红系细胞刺激了骨髓细胞的集落形成活性，这可能是通过细胞因子合成（如粒细胞-巨噬细胞集落刺激因子）介导的。
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