Ren Y, Munyer P, Kepes J J, Cansler M, Collins J, Gayed B, Tawfik O
Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City 66160, USA.
Appl Immunohistochem Mol Morphol. 2001 Mar;9(1):14-8.
Traditional prognostic indicators for astrocytic tumors include tumor size, type, and histologic grade. Data suggest that tumor growth fraction assessed by MIB-1 is an important predicator of survival. Cyclin A, like MIB-1, is a recently described specific marker of proliferation, detectable primarily in S phase of the cell cycle as it undergoes progression to G2 phase. Thirty-seven cases of astrocytic tumors--14 cases of World Health Organization grade 1 and 2 (low grade tumors), 8 cases of grade 3 (anaplastic astrocytoma), and 15 cases of grade 4 (glioblastoma multiforme)--were simultaneously evaluated using routine paraffin immunohistochemical methods with commercial antibodies against MIB-1 and cyclin A. The results were quantitated using a Cell Analysis System (CAS) 200 image analyzer. The mean percentage positive nuclear area for MIB-1 was 3.32% in grade 1 and 2, 19.27% in grade 3, and 24.00% in grade 4 astrocytic tumors. Cyclin A showed a similar pattern of positivity in the same cases: 2.84% in grade 1 and 2, 16.27% in grade 3, and 24.88% in grade 4 astrocytic tumors. The data suggest that both proliferation markers correlated significantly with histologic grade. Cyclin A appears to be as good an indicator of brain tumor proliferation as MIB-1. Because cyclin A is detectable primarily in the S phase of the cell cycle, the fraction of cells positive for cyclin A should allow for a more accurate indicator of tumor progression.
星形细胞瘤的传统预后指标包括肿瘤大小、类型和组织学分级。数据表明，通过MIB-1评估的肿瘤生长分数是生存的重要预测指标。细胞周期蛋白A与MIB-1一样，是一种最近描述的增殖特异性标志物，主要在细胞周期的S期检测到，此时细胞正进入G2期。使用抗MIB-1和细胞周期蛋白A的商业抗体，通过常规石蜡免疫组织化学方法同时评估了37例星形细胞瘤病例，其中14例为世界卫生组织1级和2级（低级别肿瘤），8例为3级（间变性星形细胞瘤），15例为4级（多形性胶质母细胞瘤）。使用细胞分析系统（CAS）200图像分析仪对结果进行定量分析。在1级和2级星形细胞瘤中，MIB-1的平均阳性核面积百分比为3.32%，3级为19.27%，4级为24.00%。在相同病例中，细胞周期蛋白A显示出类似的阳性模式：1级和2级为2.84%，3级为16.27%，4级星形细胞瘤为24.88%。数据表明，这两种增殖标志物均与组织学分级显著相关。细胞周期蛋白A似乎与MIB-1一样，是脑肿瘤增殖的良好指标。由于细胞周期蛋白A主要在细胞周期的S期检测到，因此细胞周期蛋白A阳性细胞的比例应该能够更准确地指示肿瘤进展。
