Vercauteren I, Van Der Schueren E, Van Montagu M, Gheysen G
Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, Belgium.
Mol Plant Microbe Interact. 2001 Mar;14(3):288-99. doi: 10.1094/MPMI.2001.14.3.288.
In the compatible interaction between Arabidopsis thaliana and the endoparasitic nematode Meloidogyne incognita, galls are formed on the roots of the host plant. Differential display was used to identify alterations of gene expression in young A. thaliana root galls caused by M. incognita. Six genes were confirmed as plant genes by DNA gel blot hybridizations. Significant homology was found with a trypsin inhibitor, peroxidase, mitochondrial uncoupling protein, endomembrane protein, 20S proteasome alpha-subunit, and diaminopimelate decarboxylase. The cellular and temporal expression of each of the six genes was analyzed by mRNA in situ hybridizations.
在拟南芥与内寄生线虫南方根结线虫的亲和互作过程中，寄主植物根上会形成虫瘿。利用差异显示技术来鉴定由南方根结线虫引起的拟南芥幼根虫瘿中基因表达的变化。通过DNA凝胶印迹杂交确认了6个基因是植物基因。发现它们与一种胰蛋白酶抑制剂、过氧化物酶、线粒体解偶联蛋白、内膜蛋白、20S蛋白酶体α亚基和二氨基庚二酸脱羧酶具有显著同源性。通过mRNA原位杂交分析了这6个基因各自的细胞和时间表达情况。
