构建于根癌农杆菌可转化载体中的百脉根细菌人工染色体文库。
A bacterial artificial chromosome library of Lotus japonicus constructed in an Agrobacterium tumefaciens-transformable vector.
作者信息
Men A E, Meksem K, Kassem M A, Lohar D, Stiller J, Lightfoot D, Gresshoff P M
机构信息
Department of Botany, University of Queensland, Brisbane, Australia.
出版信息
Mol Plant Microbe Interact. 2001 Mar;14(3):422-5. doi: 10.1094/MPMI.2001.14.3.422.
PMID:11277441
Abstract
We constructed a BAC library of the model legume Lotus japonicus with a 6-to 7-fold genome coverage. We used vector PCLD04541, which allows direct plant transformation by BACs. The average insert size is 94 kb. Clones were stable in Escherichia coli and Agrobacterium tumefaciens.
摘要
我们构建了一个覆盖模型豆科植物百脉根基因组6至7倍的细菌人工染色体（BAC）文库。我们使用了载体PCLD04541，它允许通过BAC进行直接植物转化。平均插入片段大小为94 kb。克隆在大肠杆菌和根癌农杆菌中是稳定的。
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