Keller M, Miller A D
Imperial College Genetic Therapies Centre, Department of Chemistry, Imperial College of Science, Technology and Medicine, South Kensington, UK.
Bioorg Med Chem Lett. 2001 Mar 26;11(6):857-9. doi: 10.1016/s0960-894x(01)00085-3.
The solid-phase peptide synthesis of a reportedly inaccessible peptide sequence of chaperonin 60.1 (195-219) is described using oxazolidine containing dipeptide building blocks ('pseudo-proline' dipeptide units). Two attempts at the synthesis of the chaperonin 60.1 sequence are outlined using one and two pseudo-proline units, respectively, and these results are compared with the outcome of an ordinary stepwise (double) coupling procedure. The only successful synthesis is that combining two pseudo-proline building blocks.
本文描述了使用含恶唑烷的二肽构建模块（“假脯氨酸”二肽单元）对伴侣蛋白60.1（195 - 219）中据报道难以合成的肽序列进行固相肽合成。分别概述了使用一个和两个假脯氨酸单元合成伴侣蛋白60.1序列的两次尝试，并将这些结果与普通逐步（双）偶联程序的结果进行了比较。唯一成功的合成是结合两个假脯氨酸构建模块的合成。
