一种可提高寡核苷酸双链体稳定性的2'-酰基酰胺帽。
A 2'-acylamido cap that increases the stability of oligonucleotide duplexes.
作者信息
Kryatova O P, Connors W H, Bleczinski C F, Mokhir A A, Richert C
机构信息
Departments of Chemistry, Tufts University, Medford, Massachusetts 02155, and University of Constance, D-78457 Konstanz, Germany.
出版信息
Org Lett. 2001 Apr 5;3(7):987-90.
[structure: see text]. Reported here is the synthesis of oligonucleotides containing a 2'-acylamido-2'-deoxyuridine residue at their 3'-terminus. Compared to control sequences bearing a thymidine residue, the quinolone-capped duplexes give higher UV melting points. In the case of (5'-ACGCGU-NA-2')2, where NA denotes a nalidixic acid residue, the melting point increase is up to 22 degrees C over that of (ACGCGT)2.
[结构：见正文]。本文报道了在其3'-末端含有2'-酰氨基-2'-脱氧尿苷残基的寡核苷酸的合成。与带有胸腺嘧啶残基的对照序列相比，喹诺酮封端的双链体具有更高的紫外熔点。在(5'-ACGCGU-NA-2')2的情况下，其中NA表示萘啶酸残基，其熔点比(ACGCGT)2高出22摄氏度。
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