Queiroz J A, Tomaz C T, Cabral J M
Departamento de Química, Universidade da Beira Interior, Covilha 6201-001, Portugal.
J Biotechnol. 2001 May 4;87(2):143-59. doi: 10.1016/s0168-1656(01)00237-1.
In this article, an overview of hydrophobic interaction chromatography (HIC) of proteins is given. After a brief description of protein hydrophobicity and hydrophobic interactions, we present the different proposed theories for the retention mechanism of proteins in HIC. Additionally, the main parameters to consider for the optimization of fractionation processes by HIC and the stationary phases available were described. Selected examples of protein fractionation by HIC are also presented.
本文对蛋白质的疏水作用色谱法（HIC）进行了概述。在简要描述蛋白质疏水性和疏水相互作用之后，我们介绍了关于蛋白质在HIC中保留机制的不同提出的理论。此外，还描述了通过HIC优化分级分离过程时要考虑的主要参数以及可用的固定相。还给出了通过HIC进行蛋白质分级分离的选定示例。
