Park K U, Kim S H, Suh C, Kim S, Lee S J, Park J S, Cho H J, Kim K W, Lee K, Kim H J, Park J, Joo Min Y, Kim J G, Kim T, Lee J H, Kim S B, Kim S W, Lee K H, Lee J S, Kim W K, Park C J, Chi H S
Department of Medicine, Dongguk University College of Medicine, Kyongju, Korea.
Am J Hematol. 2001 May;67(1):42-7. doi: 10.1002/ajh.1074.
The yield of stem cell collection after mobilization is crucial for autologous peripheral blood stem cell (PBSC) transplantation. Quantitative determinations of CD34(+) cells using flow cytometry or stem cell culture have been used, but these methods require much time, technical experience, and expensive reagents. The automated hematology analyzer (Sysmex SE-9000trade mark, TOA, Japan) equipped with the Immature Information (IMI) channel for immature myeloid cells can detect IMI(+) cells within 90 sec. Detection is made possible by the combination of a special reagent system and direct current/radiofrequency biosensors. We studied the relation of IMI(+) cells and variable cell counts with CD34(+) cell yield in autologous stem cell harvest. In a series of 32 patients (median age, 44 years; M:F = 11:21), 184 leukaphereses were performed after mobilization regimens with chemotherapy and G-CSF or G-CSF alone. Full blood cell counts were enumerated on peripheral blood (PB) samples taken prior to each leukapheresis. Mononuclear cell (MNC) and IMI(+) cell counts by automated hematology analyzer and flow cytometry based CD34(+) cell yield were measured on the harvested product. The relationship among PB white blood cells (WBC), PB monocytes, IMI(+) cells, MNC, and CD34(+) cell yield in a single leukapheresis was estimated by Pearson correlation analysis. PB WBC count showed no correlation with CD34(+) cell yield in a single leukapheresis (r = 0.02, P = 0.81). PB monocyte count showed a weak correlation (r = 0.21, P = 0.01) and MNC in harvest also showed a weak correlation (r = 0.36, P = 0.0001) with CD34(+) cell yield. In contrast, CD34(+) cell yield correlated well with IMI(+) cell count (r = 0.68, P = 0.0001), and data could be fitted by a linear regression equation, y = 0.330 + 0.974x. IMI(+) cell assay by the automated hematology analyzer correlated well with the CD34(+) cell yield in a mobilized autologous stem cell harvest. The IMI(+) cell count might be used as a simple and efficient indicator of blood stem cell mobilization and collection.
动员后干细胞采集的产量对于自体外周血干细胞（PBSC）移植至关重要。已采用流式细胞术或干细胞培养对CD34(+)细胞进行定量测定，但这些方法需要大量时间、技术经验和昂贵的试剂。配备用于未成熟髓细胞的未成熟信息（IMI）通道的自动血液分析仪（Sysmex SE - 9000商标，日本东亚）可在90秒内检测IMI(+)细胞。通过特殊试剂系统和直流/射频生物传感器的组合实现检测。我们研究了自体干细胞采集过程中IMI(+)细胞和可变细胞计数与CD34(+)细胞产量之间的关系。在一系列32例患者（中位年龄44岁；男:女 = 11:21）中，在采用化疗和G - CSF或仅G - CSF的动员方案后进行了184次白细胞分离术。在每次白细胞分离术前采集的外周血（PB）样本上进行全血细胞计数。对采集产物进行自动血液分析仪检测单核细胞（MNC）和IMI(+)细胞计数以及基于流式细胞术的CD34(+)细胞产量测定。通过Pearson相关分析估计单次白细胞分离术中PB白细胞（WBC）、PB单核细胞、IMI(+)细胞、MNC和CD34(+)细胞产量之间的关系。单次白细胞分离术中PB WBC计数与CD34(+)细胞产量无相关性（r = 0.02，P = 0.81）。PB单核细胞计数显示弱相关性（r = 0.21，P = 0.01），采集物中的MNC与CD34(+)细胞产量也显示弱相关性（r = 0.36，P = 0.0001）。相比之下，CD34(+)细胞产量与IMI(+)细胞计数相关性良好（r = 0.68，P = 0.0001），数据可由线性回归方程y = 0.330 + 0.974x拟合。自动血液分析仪检测的IMI(+)细胞测定与动员的自体干细胞采集中的CD34(+)细胞产量相关性良好。IMI(+)细胞计数可作为血液干细胞动员和采集的简单有效指标。
